163,653 results

1. PB-TAC-ERN

   Plasmid

   #80475

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3_cAMPFIRE-L

   Plasmid

   #182279

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-L (cAMP sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CD3 delta YFP

   Plasmid

   #11951

   Depositor

   Nico Dantuma

   Article

   Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

   Insert

   CD3delta-YFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Myo1b

   Plasmid

   #187362

   Purpose

   Expresses rat myosin 1b isoform b labelled with EGFP

   Depositor

   Evelyne Coudrier

   Article

   Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

   Insert

   myosin 1b isoform b (Myo1b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mApple-Alpha-V-Integrin-25

   Plasmid

   #54866

   Purpose

   Localization: Focal Adhesions, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   Alpha-V Integrin (ITGAV Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74009

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5x UAS
   Cas9
   T2A
   GFP
   U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJA179

   Plasmid

   #128537

   Purpose

   Expresses anti-Prunus dulcis scFv Ab

   Depositor

   Marcos Alcocer

   Article

   de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

   Insert

   scFv Ab clone PD1F6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac_His-ZZ-TEV-Lis1-SNAPf

   Plasmid

   #133073

   Purpose

   Plasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tag

   Depositor

   Simon Bullock, Andrew Carter

   Article

   Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

   Insert

   Lis1 (PAFAH1B1 Human)
   

   Use

   Tags

   8x His, Linker-TEV-linker_TEV, SNAPf, and ZZ

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tornado-RNA sensor for MALAT-1

   Plasmid

   #185406

   Purpose

   circular RNA-scaffolded sensing modules for MALAT-1

   Depositor

   Jian-Hui Jiang

   Article

   Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

   Insert

   circular RNA-scaffolded sensing modules
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   U6

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMega-MaPylRS

    Plasmid

    #200225

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    proK-lacO and tacI

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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