We narrowed to 17,777 results for: URE

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pHH0103_CASKIN2-1/1

Plasmid

#91261

Purpose

Protein expression and purification of human SH3 domain construct CASKIN2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CASKIN2-1/1 (CASKIN2 Human)

Expression

Bacterial

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pMGF130

Plasmid

#96971

Purpose

S. pombe lem2 knockout cassette with hygro resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

lem2 (lem2 Fission Yeast)

Expression

Bacterial

Mutation

whole ORF deletion and replaced with hygro resist…

Promoter

N/A

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMGF169

Plasmid

#96996

Purpose

ish1 mCherry insertion cassette with G418 resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + G418 …

Promoter

N/A

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pet151D-TOPO-BROX 1-401

Plasmid

#89866

Purpose

Expresses human BROX residues 1-401 in bacteria; internal ID WISP11-297

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

BROX (BROX Human)

Tags

His Tag, V5 tag

Expression

Bacterial

Mutation

expresses only residues 1-401

Promoter

T7

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-EGFP L1-R5

Plasmid

#84391

Purpose

Multisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Gateway

Tags

EGFP

Expression

Mammalian

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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KCC4_HUMAN_D0

Plasmid

#79744

Purpose

This plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC4 (SLC12A7 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KS6A1_HUMAN_D1

Plasmid

#79720

Purpose

This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KS6A1 (RPS6KA1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK10_HUMAN_D0

Plasmid

#79722

Purpose

This plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK10 (STK10 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
exon K-renilla

Plasmid

#81007

Purpose

luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

Depositor

Richard Baines

Article

Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

Insert

paralytic (para Fly)

Tags

Renilla luciferase

Expression

Insect

Mutation

spanning exon 24 to exon 26, termination codon in…

Promoter

P-AC5 (Actin 5C)

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1.3

Plasmid

#46682

Purpose

Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1.3 (MIR16-1 Synthetic, Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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