Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 8,387 results for: BLI;

Showing: 4721 - 4760 of 8387 results

  2. pAAVS hOPM

    Plasmid
    #112498
    Purpose
    Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in AAVS locus.
    Depositor
    Bruce Conklin
    Insert
    OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    112498-plasmid-map-sequence-id-232755

    Enlarge

  3. pLV Agpat2-V5

    Plasmid
    #175159
    Purpose
    Lentiviral expression of mouse Agpat2-V5
    Depositor
    Larry Gerace
    Insert
    Agpat2 (Agpat2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    175159-plasmid-map-sequence-id-352271

    Enlarge

  4. PANK1

    Plasmid
    #32871
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK1 (PANK1 Human)
    Use
    Tags
    His tag (with Thrombin cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 231-597 only
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pCMV-T7-ABEmax-SpG

    Plasmid
    #195278
    Purpose
    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP
    Depositor
    Carsten Lederer, Petros Patsali
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195278-plasmid-map-sequence-id-394868

    Enlarge

  8. DVL2

    Plasmid
    #38876
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DVL2 (DVL2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pLV-Enh eGFP Reporter-huKC2-IGR21

    Plasmid
    #59456
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the human keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    huKrt1 3’intergenic region
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pUltra-hot-H2B-mTagBFP2

    Plasmid
    #161792
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Multicistronic
    Tags
    mCherry and mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161792-plasmid-map-sequence-id-316815

    Enlarge

  11. p2Bac.HA.p52

    Plasmid
    #39219
    Depositor
    Aziz Sancar
    Insert
    p52 (GTF2H4 Human)
    Use
    Tags
    HA
    Expression
    Insect
    Mutation
    Promoter
    Pp10
    Available since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  12. pLV Cnep1r1-V5

    Plasmid
    #175133
    Purpose
    Lentiviral expression of mouse Cnep1r1-V5
    Depositor
    Larry Gerace
    Insert
    Cnep1r1 (Cnep1r1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175133-plasmid-map-sequence-id-352020

    Enlarge

  14. pKG187

    Plasmid
    #63077
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FAAP24, IMAGE clone 3659284 (FAAP24 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    63077-plasmid-map-sequence-id-134680

    Enlarge

  15. HOXC9 (murine) HIS-tag pET

    Plasmid
    #8559
    Depositor
    Corey Largman
    Insert
    HOXC9 (Hoxc9 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    makes protein by Western
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  16. SMYD3

    Plasmid
    #32048
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SMYD3 (SMYD3 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. 4P7A

    Plasmid
    #61954
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pcDNA5/FRT/TO SBBP-CDK1 WT (2007)

    Plasmid
    #61847
    Purpose
    mammalian expression of CDK1 WT, used for Ips
    Depositor
    Jonathon Pines
    Insert
    CDK1 (CDK1 Human)
    Use
    Tags
    SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. 4MTL

    Plasmid
    #60100
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL21C:PBC002-E06:C229066 (METTL21C Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. isoform PKC Zeta 2 (JCE863)

    Plasmid
    #182028
    Purpose
    yeast expression of mouse PKC Zeta isoform 2 with yeast PKC1 promoter and ADH1 terminator
    Depositor
    Juan Carlos Igual
    Insert
    PKC Zeta isoform 2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    yeast PKC1 promoter (-600 to -1)
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    182028-plasmid-map-sequence-id-362000

    Enlarge

  22. PRDX1A-c001

    Plasmid
    #175473
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX1 (PRDX1 Human)
    Use
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    175473-plasmid-map-sequence-id-353180

    Enlarge

  23. pENTRY4_HuB

    Plasmid
    #65752
    Purpose
    entry vector with HuB
    Depositor
    Thomas Tuschl
    Insert
    ELAVL2 (ELAVL2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    65752-plasmid-map-sequence-id-117917

    Enlarge

  24. pLEX307-CTSL-G418

    Plasmid
    #158468
    Purpose
    Constitutive lentiviral expression of human CTSL.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSL (CTSL Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158468-plasmid-map-sequence-id-308165

    Enlarge

  25. pHyg-PCUP1-AID*(N)

    Plasmid
    #99529
    Purpose
    N-terminal AID* degron cassette with CUP1 promoter and HygR marker for S. cerevisiae
    Depositor
    Helle Ulrich
    Insert
    IAA17(71-117) (AXR3 Mustard Weed)
    Use
    Pcr template for yeast tagging cassette
    Tags
    Expression
    Mutation
    Promoter
    CUP1
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99529-plasmid-map-sequence-id-192013

    Enlarge

  26. pGEX-6P-Cdh1wt (570)

    Plasmid
    #39877
    Depositor
    Jonathon Pines
    Insert
    FZR1 (FZR1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. PA-mCherry-Alpha-5-Integrin-12

    Plasmid
    #62790
    Purpose
    Localization: Focal Adhesions, Excitation:400 / 504, Emission:515 / 517
    Depositor
    Michael Davidson
    Insert
    Alpha5Integrin (ITGAV Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  28. ENO3

    Plasmid
    #39072
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ENO3 (ENO3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)

    Plasmid
    #44081
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44081-plasmid-map-sequence-id-61675

    Enlarge

  30. pSpot-Tag-Lamin

    Plasmid
    #117062
    Purpose
    For mammalian expression of lamin B1 with N-terminal Spot-Tag
    Depositor
    Felix Hartlepp
    Insert
    Lamin B1 (LMNB1 Human)
    Use
    Tags
    Spot-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117062-plasmid-map-sequence-id-224387

    Enlarge

  31. pENTRY4_HuD

    Plasmid
    #65754
    Purpose
    entry vector with HuD
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65754-plasmid-map-sequence-id-114982

    Enlarge

  32. p-dCas9-p300-S1396R-S1397R-Hygro

    Plasmid
    #104419
    Purpose
    transient expression of dCas9-dp300 fusion protein
    Depositor
    Stephan Beck
    Insert
    dp300
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    S1396R-S1397R
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104419-plasmid-map-sequence-id-204303

    Enlarge

  34. pKG402

    Plasmid
    #69760
    Purpose
    Retroviral expression of CENP-T aa 256-561
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 256-561 (CENPT Human)
    Use
    Retroviral
    Tags
    mCherry-TEV-S
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. pIC84

    Plasmid
    #63062
    Purpose
    Retroviral expression of EGFP-TEV-S-tag hCENP-H for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    hCENP-H, IMAGE clone 3451767 (CENPH Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63062-plasmid-map-sequence-id-104377

    Enlarge

  37. pPyCAG-Nanog(K140A)-IP

    Plasmid
    #62998
    Purpose
    Expresses Mouse Nanog K140A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K140A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Lysine 140 (141 AA in Mouse Protein) to A…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62998-plasmid-map-sequence-id-104601

    Enlarge

  38. pTF267 (pFA6a-TEV-6xGly-12xHis-KanMX)

    Plasmid
    #44093
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44093-plasmid-map-sequence-id-61671

    Enlarge

  39. pAAV asyn S129E

    Plasmid
    #36068
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S129E (SNCA Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S129E
    Promoter
    CMV
    Available since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only

  40. pLV H2afy-V5

    Plasmid
    #175122
    Purpose
    Lentiviral expression of mouse Macroh2a1-V5
    Depositor
    Larry Gerace
    Insert
    Macroh2a1 (Macroh2a1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175122-plasmid-map-sequence-id-351930

    Enlarge

Showing: 4721 - 4760 of 8387 results