We narrowed to 5,211 results for: golden gate

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pSLQ14902

Plasmid

#239277

Purpose

Expresses gG2 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

Depositor

Stanley Qi

Publication

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

PspCas13b-hGAPDH-gRNA2 (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_Cas9d_Plasmid

Plasmid

#239445

Purpose

Expression plasmid codon-optimized for E. coli

Depositor

David Taylor

Publication

Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

Insert

Cas9d

Expression

Bacterial

Promoter

T7

Available since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCA3)

Plasmid

#235064

Purpose

Expresses Tad-CBE(TCA3) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCA3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCB094 FoMV-sgLw

Plasmid

#234816

Purpose

To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbLw (T-DNA)

Depositor

Daniel Voytas

Publication

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

gRNA targeting SbLw

Expression

Plant

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCB092 FoMV-sgMgCh

Plasmid

#234815

Purpose

To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbMgCh (T-DNA)

Depositor

Daniel Voytas

Publication

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

gRNA targeting SbMgCh

Expression

Plant

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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Reckleen_3plus_sgRNA_ptet_pos4

Plasmid

#233459

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Publication

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, ColE1 ori, and KanR.

Use

CRISPR

Available since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3_multieditdropout

Plasmid

#233458

Purpose

Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

Depositor

Anke Becker

Publication

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4

Use

CRISPR

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-mGL

Plasmid

#232768

Purpose

IVT template for making modRNA encoding mGreenLantern

Depositor

Kate Galloway

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mGreenLantern

Use

In vitro transcription

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAGC-Lb12-P1Bb

Plasmid

#231387

Purpose

For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

Depositor

Qi-Jun Chen

Publication

Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

Insert

U6-29

Use

CRISPR

Expression

Plant

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227890

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Publication

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET29-neoLux1.2

Plasmid

#229952

Purpose

Bacterial expression for de novo luciferase, neoLux1.2

Depositor

Andy Yeh

Publication

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

neoLux1.2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pR-mAmetrine

Plasmid

#228598

Purpose

Mammalian expression of mAmetrine, for MuSIC barcode construction

Depositor

Marc Birtwistle

Publication

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mAmetrine

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW91013

Plasmid

#222450

Purpose

Level 1, Position 3 containing CBS4-miniDFR::RUBY::OCS.

Depositor

Chih-Hang Wu

Publication

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR::RUBY::OCS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW91015

Plasmid

#222455

Purpose

Level 1, Position 2 containing Mas::CUP2-VP16::NOS.

Depositor

Chih-Hang Wu

Publication

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

Mas::CUP2-VP16::NOS (CUP2 )

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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scFv-HaloTag-iLID

Plasmid

#227390

Purpose

Adhesion module, scFv binds SunTag

Depositor

Clifford Brangwynne

Publication

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

scFv-HaloTag-iLID

Use

Lentiviral

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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miRFP670-TRF1

Plasmid

#227388

Purpose

Label telomere, no adhesion

Depositor

Clifford Brangwynne

Publication

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric miRFP (TERF1 Human)

Use

Lentiviral

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHEE-R-hyg9si

Plasmid

#222832

Purpose

CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2483 (pSpy1C)

Plasmid

#220248

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

Depositor

Emmanuelle Charpentier

Publication

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAGT9054

Plasmid

#219430

Purpose

Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS

Depositor

Tom Schreiber

Publication

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)

Use

CRISPR; Moclo compatible level 1 module (position…

Tags

PapE-4LF2

Expression

Plant

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-ELP[V]60

Plasmid

#219406

Purpose

(VPGVG)60

Depositor

Danielle Mai

Publication

Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

Insert

ELP[V]60

Tags

6xHis

Expression

Bacterial

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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