We narrowed to 5,125 results for: golden gate
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Plasmid#232768PurposeIVT template for making modRNA encoding mGreenLanternDepositorInsertmGreenLantern
UseIn vitro transcriptionAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAGC-Lb12-P1Bb
Plasmid#231387PurposeFor crRNA cloning with LbCas12a vectors for genome editing in ArabidopsisDepositorInsertU6-29
UseCRISPRExpressionPlantAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_CAG-mRuby2
Plasmid#229801PurposeBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterCAGAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227890PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertTOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29-neoLux1.2
Plasmid#229952PurposeBacterial expression for de novo luciferase, neoLux1.2DepositorInsertneoLux1.2
TagsHis-tagExpressionBacterialPromoterT7Available SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCHW91013
Plasmid#222450PurposeLevel 1, Position 3 containing CBS4-miniDFR::RUBY::OCS.DepositorInsertCBS4-miniDFR::RUBY::OCS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3478 PB-PEmax-mTagBFP2
Plasmid#226664PurposePiggyBac cargo vector encoding PEmax and mTagBFP2DepositorInsertsPEmax
mTagBFP2
UseCRISPRExpressionMammalianPromoterCAG and UBCAvailable SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
scFv-HaloTag-iLID
Plasmid#227390PurposeAdhesion module, scFv binds SunTagDepositorInsertscFv-HaloTag-iLID
UseLentiviralAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
miRFP670-TRF1
Plasmid#227388PurposeLabel telomere, no adhesionDepositorInsertTelomeric miRFP (TERF1 Human)
UseLentiviralAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-hyg9si
Plasmid#222832PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT9054
Plasmid#219430PurposeTranscriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCSDepositorInsertLB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
UseCRISPR; Moclo compatible level 1 module (position…TagsPapE-4LF2ExpressionPlantAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ELP[V]60
Plasmid#219406Purpose(VPGVG)60DepositorInsertELP[V]60
Tags6xHisExpressionBacterialAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOP01-UTC-7.0
Plasmid#216625PurposeNanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test constructDepositorInsertT7p-5'g10-NanoLuc-3'TMV
UseSynthetic BiologyPromoterT7Available SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
LYS-Leu2
Plasmid#218114PurposeIntegration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPD189 VP16-ZF37
Plasmid#138759PurposeConstitutive expression of VP16-ZF37 under the CMV promoter.DepositorInsertVP16-ZF37
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPS0378
Plasmid#133229PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH183
Plasmid#122856PurposeMS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequencesDepositorInsertMS2-modified sgRNA targeting the Arabidopsis FT promoter
UseGolden gate compatible cloning vectorAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH204
Plasmid#122860PurposeBinary expression vector containing 35S:MS2-VP64:tRBCS followed by GreenGate A-G sequences for insertion of additional modulesDepositorInsert35S:MS2-VP64:tRBCS
UseGolden gate compatible plant transformation vectorAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGM000
Plasmid#48864PurposeEmpty entry vector for creating GreenGate supermodules w/o plant resistance cassette.DepositorTypeEmpty backboneUseGolden gate compatible cloning vectorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only