We narrowed to 146,089 results for: ins;

1. pX330 _CRISPIE donor B7

   Plasmid

   #172848

   Purpose

   DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330 _CRISPIE donor B9

   Plasmid

   #172850

   Purpose

   DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSR660

   Plasmid

   #65216

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-8 cap
   

   Use

   AAV

   Tags

   Expression

   Insect

   Mutation

   Promoter

   P10 and polyhedrin

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Stat1 beta Flag pRc/CMV

   Plasmid

   #8704

   Depositor

   Jim Darnell

   Article

   Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

   Insert

   STAT1 beta (STAT1 Human)
   

   Use

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pDV06.2c_DART-VADAR_iRFP720-sensor

   Plasmid

   #200410

   Purpose

   pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   Closed-loop DART VADAR sensor detecting iRFP720
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR P4-P1R-ECFP

   Plasmid

   #48352

   Purpose

   Contributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Tomas Santalucia, Anna Planas

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   ECFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Contains a Kozak sequence. Does not contain a sto…

   Promoter

   none

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBGPa-CMV-GFP-OSBP PH domain

   Plasmid

   #58724

   Purpose

   Expresses GFP-Tagged OSBP PH domain in mammalian cells

   Depositor

   Tim Levine

   Article

   Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

   Insert

   Oxysterol-binding Protein PH domain (OSBP Human)
   

   Use

   Tags

   GFP and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pSR651

   Plasmid

   #65213

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-1 cap
   

   Use

   AAV

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10 and polyhedrin

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-HtrA2-EGFP

   Plasmid

   #14121

   Depositor

   L. Miguel Martins

   Article

   Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2007

   Availability

   Academic Institutions and Nonprofits only

10. Stat1 alpha pRC/CMV

    Plasmid

    #8690

    Depositor

    Jim Darnell

    Article

    Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

    Insert

    STAT1 alpha (STAT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pX335-EN475

    Plasmid

    #86231

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX335-EN477

    Plasmid

    #86232

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE2 GFP-Atl1

    Plasmid

    #86679

    Purpose

    Lentivirus to stably express fluorescent protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    ATL1 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET30a-Spike-E

    Plasmid

    #165096

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid

    #47550

    Purpose

    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Inserts

    Cas9
    ttTi5605 sgRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    U6 and eef-1A.1 (eft-3)

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPong_v2

    Plasmid

    #47101

    Purpose

    Expresses the Pong ORF1 and transposase from rice to allow mPing movement. Contains ORF1 and transposase driven by a native soybean promoter. Contains mPing and BAR for basta selection.

    Depositor

    Wayne Parrott

    Article

    Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

    Inserts

    mPing
    Pong Open Reading Frame 1 (ORF1)
    Pong Transposase
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Promoter

    GmUbi_AP, GmUbi_XS, and no promoter

    Available since

    Oct. 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. MBC2-null

    Plasmid

    #162749

    Purpose

    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MBC1-null

    Plasmid

    #162748

    Purpose

    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. YFVdelCMENS1-Cre

    Plasmid

    #179954

    Purpose

    pCCI vector encoding YFV-17D lacking C, prM, E and NS1 proteins but with Cre

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    YFV-17D lacking C, M, E and NS1 proteins but with Cre
    

    Use

    Transneuronal tracing

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. DEL-pHG165a

    Plasmid

    #90064

    Purpose

    LLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmid

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    DEL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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