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AAV-hSyn-RiboL1-jGCaMP8f

Plasmid

#169249

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MMP8 Wt

Plasmid

#29545

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-SwitchON-sgVRK1

Plasmid

#199638

Purpose

Tamoxifen-inducible expression of sgRNA targeting VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ Flag-RAP2A

Plasmid

#128099

Purpose

Expresses RAP2A in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

RAP2A (RAP2A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ Flag-RAGB99L

Plasmid

#128101

Purpose

Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

Ras related GTP binding B (RRAGB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Q99L (CAA to CTA)

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MMP8 (P78S)

Plasmid

#29546

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

P78S

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MMP8 (G104R)

Plasmid

#29548

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

G104R

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro

Plasmid

#212675

Purpose

Express tagged hRb phosphosite mutant

Depositor

Tobias Meyer, Yumi Konagaya

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

Rb (RB1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

15 CDK phospho-sites are mutated to alanines, and…

Promoter

TRE

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MMP8 (E87D)

Plasmid

#29547

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E87D

Promoter

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
PCMV-Tag4A-MMP8 (E138Q)

Plasmid

#29549

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E138Q

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
shCD44-2 pRRL

Plasmid

#19123

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against CD44 (CD44 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 2, 2008

Availability

Academic Institutions and Nonprofits only

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CD44S pBabe-puro

Plasmid

#19127

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

CD44 standard (CD44 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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CD44S pWZL-Blast

Plasmid

#19126

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

CD44 standard (CD44 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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CD44P pGL3

Plasmid

#19122

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

CD44 promoter (CD44 Human)

Use

Non viral cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#99373

Purpose

3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

(Sp)dCas9-VPR-P2A-Puro

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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shp53 pLKO.1 puro

Plasmid

#19119

Purpose

3rd gen lentiviral vector for knocking down p53 gene expression

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p53 (TP53 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

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pLenti-hACE2-hygro

Plasmid

#161758

Purpose

Expresses human ACE2 in mammalian cells

Depositor

Neville Sanjana

Article

Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

Insert

Human ACE2 (ACE2 Human)

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC33A1

Plasmid

#132264

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC33A1 (SLC33A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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p80-Katanin-mNeonGreen

Plasmid

#191762

Purpose

Expression of mNeonGreen fused to p80-Katanin

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

p80-Katanin-mNeonGreen (KATNB1 Human)

Use

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Ribo-jGCaMP8s

Plasmid

#167572

Purpose

DEPRECATED: Please see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV1

Insert

Ribo-GCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge