3,636 results

1. pMT-TRAF6-V5-His

   Plasmid

   #63752

   Purpose

   Expresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   TRAF6 (Traf6 Fly)
   

   Tags

   V5-His

   Expression

   Insect

   Promoter

   pMT

   Available Since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. AByG

   Plasmid

   #111083

   Purpose

   Integrates marker gene onto D. melanogaster Y chromosome

   Depositor

   Omar Akbari

   Article

   Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

   Inserts

   3xP3 promoter
   tdTomato
   Gypsy insulator
   CTCF insulator
   Y homology arm left
   Y homology arm right
   

   Expression

   Insect

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT-Flag-RELISH

   Plasmid

   #63750

   Purpose

   Expresses drosophila RELISH tagged with a Flag epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   Relish (Rel Fly)
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   pMT

   Available Since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJFRC7-UAS-NPRR_ANP

   Plasmid

   #133243

   Purpose

   Fluorescent reporter for neuropeptide release imaging

   Depositor

   David Anderson

   Article

   Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

   Insert

   rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
   

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 10XUAS-flp-DD

   Plasmid

   #124777

   Purpose

   Expresses flippase fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

   Depositor

   Jing-W Wang

   Article

   Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

   Insert

   TMP-inducible destabilized FLP (FLP-DD)
   

   Tags

   DD

   Expression

   Insect

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PRE-Hsp70BbCas9_1.2

   Plasmid

   #190796

   Purpose

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Expression

   Insect

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ1272

   Plasmid

   #44486

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, MCS
   

   Use

   Multiple cloning site vector

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUASp YFP Ras85D WT

   Plasmid

   #52242

   Purpose

   Drosophila expression of YFP-Ras85D

   Depositor

   Matthew Scott

   Insert

   Ras85D (Ras85D Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Promoter

   GAL4

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

9. K560GFP_17NL

   Plasmid

   #129767

   Purpose

   Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Tags

   GFP and His6

   Expression

   Bacterial

   Mutation

   DAL insert before A345

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a_DmKHC[1-421]-SNAP-6xHis

    Plasmid

    #196975

    Purpose

    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

    Depositor

    Lukas Kapitein

    Article

    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Tags

    6xHis and SNAP

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME18ST-d5-HT1B

    Plasmid

    #53108

    Purpose

    Expression of Drosophila 5-HT1B

    Depositor

    Leslie Vosshall

    Article

    Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

    Insert

    5HT1B (5-HT1B Fly)
    

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pME18ST-d5-HT2A

    Plasmid

    #53109

    Purpose

    Expression of Drosophila 5-HT2A

    Depositor

    Leslie Vosshall

    Article

    Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

    Insert

    5HT2A (5-HT2 Fly)
    

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pME18ST-d5-HT1A

    Plasmid

    #53107

    Purpose

    Expression of Drosophila 5-HT1A

    Depositor

    Leslie Vosshall

    Article

    Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

    Insert

    5HT1A (5-HT1A Fly)
    

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid

    #126472

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc5.1B-EGFP-DmG3BP_G

    Plasmid

    #146349

    Purpose

    Insect Expression of DmG3BP

    Depositor

    Elisa Izaurralde

    Insert

    DmG3BP (rin Fly)
    

    Expression

    Insect

    Mutation

    V632G compared to AF231031.1, exact match to NP_5…

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGC(2.1)-U6.3

    Plasmid

    #170516

    Purpose

    PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(2.1)-pU6.3
    

    Use

    Pcr template vector for amplifying grnacore(2.1)-…

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDmAlfaTub84b

    Plasmid

    #165781

    Purpose

    Drosophila Alpha Tubulin promoter

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    AlfaTub84b
    

    Expression

    Insect

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJWZ6

    Plasmid

    #21744

    Depositor

    David Sherwood

    Article

    Ziel et al Nat Cell Biol. 2009 Feb . 11(2):183-9.

    Insert

    mCherry::dMoeABD
    

    Use

    Bacterial propagation

    Tags

    mCherry

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. p64PLA5

    Plasmid

    #67284

    Purpose

    6/4 photolyase gene from Drosophila in pFastbac vector, intermediate step in insect cell expression

    Depositor

    Aziz Sancar

    Article

    Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.

    Insert

    6/4 photolyase from Drosophila
    

    Use

    Intermediate for insect expression

    Tags

    Flag

    Mutation

    none known

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

20. KHBD00476

    Plasmid

    #39585

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5838 (Dref Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only