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Showing: 441 - 480 of 3490 results

  1. pSB700-mouse-TTN-Cerulean

    Plasmid
    #128325
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against mouse TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    128325-plasmid-map-sequence-id-255042

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  2. pML107-KAE1

    Plasmid
    #232891
    Purpose
    Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    KAE1 gRNA (KAE1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232891-plasmid-map-sequence-id-470392

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  3. pML107-AVO1

    Plasmid
    #232889
    Purpose
    Plasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    AVO1 gRNA (AVO1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232889-plasmid-map-sequence-id-462277

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  4. pML107-YFR054C

    Plasmid
    #232900
    Purpose
    Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    YFR054C gRNA (YFR054C Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232900-plasmid-map-sequence-id-462143

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  5. pML107-SRP14

    Plasmid
    #232896
    Purpose
    Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    SRP14 gRNA (SRP14 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232896-plasmid-map-sequence-id-462141

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  6. pML107-VHT1

    Plasmid
    #232898
    Purpose
    Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    VHT1 gRNA (VHT1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232898-plasmid-map-sequence-id-462142

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  7. pML107-INO80

    Plasmid
    #232890
    Purpose
    Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    INO80 gRNA (INO80 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232890-plasmid-map-sequence-id-462082

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  8. pNSU299_MET16

    Plasmid
    #166091
    Purpose
    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    MET16 (MET16 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166091-plasmid-map-sequence-id-329609

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  9. pML107-NET1

    Plasmid
    #232893
    Purpose
    Plasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    NET1 gRNA (NET1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232893-plasmid-map-sequence-id-470393

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  10. pML107-SAM50

    Plasmid
    #232895
    Purpose
    Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    SAM50 gRNA (SAM50 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232895-plasmid-map-sequence-id-470389

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  11. pML104-HygMx4-URA3-2

    Plasmid
    #232885
    Purpose
    Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    URA3 gRNA (URA3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232885-plasmid-map-sequence-id-462211

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  12. pML107-ZIM17

    Plasmid
    #232901
    Purpose
    Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    ZIM17 gRNA (ZIM17 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232901-plasmid-map-sequence-id-462145

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  13. pAG1guide_RUBY_EggCas9

    Plasmid
    #225983
    Purpose
    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants
    Depositor
    Imran Siddiqi
    Insert
    Guide RNA against AtAGAMOUS1
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35s promoter to Drive RUBY and DD45p to drive Cas9
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225983-plasmid-map-sequence-id-448020

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  14. pCMV5 HA-TGFBRII

    Plasmid
    #19158
    Depositor
    Joan Massague
    Insert
    Transforming growth factor, beta receptor II (TGFBR2 Human)
    Use
    Tags
    HA (YDVPDYASL)
    Expression
    Mammalian
    Mutation
    PCR based mutagenesis
    Promoter
    Available since
    Oct. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
    19158-plasmid-map-sequence-id-364716

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  15. pX459-puro-hPKAalpha

    Plasmid
    #185379
    Purpose
    For mammalian expression of guide RNA: caccgTTTGAACGAATCAAGACCCT that targets human PKA subunit alpha
    Depositor
    Ayaz Najafov
    Insert
    PRKACA (PRKACA Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185379-plasmid-map-sequence-id-363450

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  16. HCP6

    Plasmid
    #166108
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166108-plasmid-map-sequence-id-330318

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  17. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid
    #140082
    Purpose
    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter
    Depositor
    Keith Joung
    Insert
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    Use
    In vitro transcription (mrna synthesis)
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    140082-plasmid-map-sequence-id-292704

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  18. LT3GEPIR-HDACshRNA

    Plasmid
    #220573
    Purpose
    To inducibly knockdown HDAC3 expression
    Depositor
    Sean Lee
    Insert
    HDAC3 shRNA (HDAC3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220573-plasmid-map-sequence-id-437932

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  19. pX459-puro-hCASP8

    Plasmid
    #185378
    Purpose
    For mammalian expression of guide RNA: AAGCAATCTGTCCTTCCTGA that targets human CASP8 (Caspase 8)
    Depositor
    Ayaz Najafov
    Insert
    CASP8 (CASP8 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185378-plasmid-map-sequence-id-363829

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  20. pZFP3.1.0-gDNA

    Plasmid
    #113779
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZFP3
    Depositor
    Kevin White
    Insert
    ZFP3 (ZFP3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113779-plasmid-map-sequence-id-230570

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  23. pV1081

    Plasmid
    #111425
    Purpose
    Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgADE-NT2
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111425-plasmid-map-sequence-id-212268

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  27. pWT062d

    Plasmid
    #107911
    Purpose
    W2m.2-4 plasmid. Contains H1-TetR-sgRNA B and is ppart of the CAMERA2m system for cellular recording in mammalian cells
    Depositor
    David Liu
    Article
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Insert
    H1-TetR-sgRNA B
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107911-plasmid-map-sequence-id-207321

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  28. pWT062e

    Plasmid
    #107910
    Purpose
    W2m.2-3 plasmid. Contains U6-LacI-sgRNA A and is ppart of the CAMERA2m system for cellular recording in mammalian cells
    Depositor
    David Liu
    Article
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Insert
    U6-LacI-sgRNA A
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107910-plasmid-map-sequence-id-207313

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  29. pX459-puro-hBRAF-1

    Plasmid
    #185365
    Purpose
    For mammalian expression of guide RNA: caccgACAACAGTTATTGGAATCTC that targets human BRAF
    Depositor
    Ayaz Najafov
    Insert
    BRAF (BRAF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185365-plasmid-map-sequence-id-363594

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  30. pX459-puro-hBRAF-2

    Plasmid
    #185366
    Purpose
    For mammalian expression of guide RNA: caccgAAAATCCCACAGATGTGGCA that targets human BRAF
    Depositor
    Ayaz Najafov
    Insert
    BRAF (BRAF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185366-plasmid-map-sequence-id-363457

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  33. pV1010

    Plasmid
    #111423
    Purpose
    gRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    sgADE-NT2
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111423-plasmid-map-sequence-id-212241

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Showing: 441 - 480 of 3490 results