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We narrowed to 11,031 results for: Hal

Showing: 441 - 480 of 11031 results

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Halo-ATG9A HRD

Plasmid

#203222

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG9 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-HaloTagx2

Plasmid

#177113

Purpose

Expresses Vps8-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

VPS8 (VPS8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag7_SKL

Plasmid

#175532

Purpose

Peroxysomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-SKL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFSynW SV2A HaloTag

Plasmid

#218545

Purpose

Lentiviral expression of SV2A HaloTag

Depositor

Edwin Chapman

Insert

SV2A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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UAS-Syt::7xHalo7

Plasmid

#87650

Purpose

For creating drosophila transgenics expressing UAS-Syt::7xHalo7

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Syt::7xHalo7

Use

Tags

Expression

Insect

Mutation

S265G in Syt (please see depositor's commen…

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-3HaloTag

Plasmid

#179828

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3x HaloTag

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn PP-HaloTag-SYT7alpha

Plasmid

#179714

Purpose

Lentiviral expression of mouse Syt7 with N terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_TRIB1:1-372

Plasmid

#210969

Purpose

Mammalian expression of full-length human TRIB1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

TRIB1:M1-C372 (TRIB1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-N1 Raptor Halo

Plasmid

#112754

Purpose

express Raptor-Halo

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Raptor-Halo (RPTOR Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

Plasmid

#169328

Purpose

Expression of HaloTag9 located at the microtubules of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag9 (CEP41 Human)

Use

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein gamma-12

Plasmid

#67043

Purpose

G protein gamma-12 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein gamma-12 (GNG12 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pSEMS-SDHB-Halo7Tag

Plasmid

#108973

Purpose

Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)

Depositor

Karin Busch

Article

Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

Insert

succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)

Use

Tags

Halo7-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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RepairTemplate_Smc4-mAID-HaloTAG

Plasmid

#217658

Purpose

Repair template to endogenously tag SMC4 with mAID-Halo Tag

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

SMC4-mAID-HaloTag (SMC4 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein gamma-5

Plasmid

#67039

Purpose

G protein gamma-5 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein gamma-5 (GNG5 Bovine)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

Plasmid

#169333

Purpose

Expression of HaloTag9 at the mitochondrial surface of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag9 (TOMM20 Human)

Use

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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6x Halo-EGFP- 2x PACT

Plasmid

#107265

Purpose

6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

6x Halo-EGFP- 2x PACT

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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UAS-Halo7::CAAX

Plasmid

#87645

Purpose

For creating drosophila transgenics expressing UAS-Halo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Halo7::CAAX

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTN-HaloTag_TRIB1:1-372

Plasmid

#210968

Purpose

Mammalian expression of full-length human TRIB1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

TRIB1:M1-C372 (TRIB1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCDNA5/FRT/TO_H2B_HaloTag9

Plasmid

#169334

Purpose

Nuclear expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag9 (H2BC21 Human)

Use

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein gamma-1

Plasmid

#67038

Purpose

G protein gamma-1 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein gamma-1 (GNGT1 Bovine)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBa. BDNF-Halo

Plasmid

#162718

Purpose

Mammalian expression of rat BDNF

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Brain derived neurotrophic factor (Bdnf Rat)

Use

Tags

Halo

Expression

Mammalian

Mutation

none

Promoter

Chicken Beta actin

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTC-HaloTag_MTA2:1-668

Plasmid

#211017

Purpose

Mammalian expression of full-length human MTA2. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

MTA2:M1-D668 (MTA2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTC-HaloTag_PAF1:1-531

Plasmid

#211076

Purpose

Mammalian expression of full-length human PAF1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

PAF1:M1-D531 (PAF1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRVdG-4Halo3-mCherry

Plasmid

#59327

Purpose

Expresses eNpHR 3.0-mCherry

Depositor

Ian Wickersham

Insert

eNpHR 3.0-mCherry

Use

Rabies virus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).cpSFGFP.HaloTag

Plasmid

#209662

Purpose

Non-responsive control

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.HaloTag

Use

AAV

Tags

Expression

Mutation

Promoter

GFAP

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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YIplac211-HaloTag-DRS2

Plasmid

#177103

Purpose

Expresses HaloTag-Drs2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

DRS2 (DRS2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2-elavl3-WHaloCaMP1a

Plasmid

#205312

Purpose

Expression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

Plasmid

#169326

Purpose

Expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
UAS-7xHalo7::CAAX

Plasmid

#87647

Purpose

For creating drosophila transgenics expressing UAS-7xHalo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

7xHalo7::CAAX

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
YIplac211-NHX1-HaloTagx2

Plasmid

#177107

Purpose

Expresses Nhx1-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

NHX1 (NHX1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-Erk2 donor

Plasmid

#113104

Purpose

Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

N terminal Erk2 donor region including homology arms and HaloTag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DNAJC5 (WT)-HaloTag

Plasmid

#205710

Purpose

Mammalian expression of DNAJC5 (WT)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTN-HaloTag_DCAF12:1-453

Plasmid

#210966

Purpose

Mammalian expression of full-length human DCAF12. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF12:M1-S453 (DCAF12 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
UAS-Syt::3xHalo7

Plasmid

#87649

Purpose

For creating drosophila transgenics expressing UAS-Syt::3xHalo7

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Syt::3xHalo7

Use

Tags

Expression

Insect

Mutation

S265G in Syt (please see depositor's commen…

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
YIplac211-ENT5-HaloTag

Plasmid

#177102

Purpose

Expresses Ent5-HaloTag in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ENT5 (ENT5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Double UP Halotag to mScarlet

Plasmid

#125138

Purpose

Natively produces Halotag, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC2 Dlg4-Halo KI

Plasmid

#183449

Purpose

CreOFF knock-in for PSD95-Halo (amino acid position: R721)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.3-hALDOA D34S

Plasmid

#210810

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSc polyA + Shal1

Plasmid

#16196

Depositor

Larry Salkoff

Article

Wei et al Science. 1990 May 4. 248(4955):599-603.

Insert

Drosophila melanogaster Shaker cognate l (Shal Fly)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
HaloTag-G protein gamma-7

Plasmid

#67040

Purpose

G protein gamma-7 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein gamma-7 (GNG7 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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