-
Plasmid#112314PurposeCRISPR donor plasmid to tag human transcription factor NR1D2 with GFPDepositorInsertNR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
UseCRISPRTagsExpressionMutationhg38:3:23977483:A>T, hg38:3:23977515:A>G, h…PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMAD3-donor
Plasmid#112311PurposeCRISPR donor plasmid to tag human transcription factor SMAD3 with GFPDepositorInsertSMAD3 homology arms flanking EGFP-IRES-Neo cassette (SMAD3 Human)
UseCRISPRTagsExpressionMutationhg38:15:67189762:C>T, rs11629568, rs8025774PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCYC1pr-GNSI (LEU)
Plasmid#111450PurposeGNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
UseTagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …PromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS1222
Plasmid#63791PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection markerDepositorInserteGFP-Twin-Strep tag followed by URA3
UseE. coli cloning vectorTagseGFP-Twin-Strep tagExpressionMutationPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJF5
Plasmid#139031PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInsertgfp (no STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJF6
Plasmid#139040PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertgfp (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNM3689
Plasmid#154111PurposeminiMos single component RMCE landing site vectorDepositorInsertloxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
UseMinimosTagsExpressionMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNM3749
Plasmid#154108PurposeloxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3
UseCre/Lox; RmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBALU1
Plasmid#69316PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfpDepositorInsertGFPint-FgF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pATF4-donor
Plasmid#113805PurposeCRISPR donor plasmid to tag human transcription factor ATF4 with GFPDepositorInsertATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
UseCRISPRTagsExpressionMutationrs140428747PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOXF2-donor
Plasmid#113816PurposeCRISPR donor plasmid to tag human transcription factor FOXF2 with GFPDepositorInsertFOXF2 homology arms flanking EGFP-IRES-Neo cassette (FOXF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pESR1-donor
Plasmid#112337PurposeCRISPR donor plasmid to tag human transcription factor ESR1 with GFPDepositorInsertESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pARNTL-donor
Plasmid#112384PurposeCRISPR donor plasmid to tag human transcription factor ARNTL with GFPDepositorInsertARNTL homology arms flanking EGFP-IRES-Neo cassette (BMAL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2C-donor
Plasmid#113824PurposeCRISPR donor plasmid to tag human transcription factor TFAP2C with GFPDepositorInsertTFAP2C homology arms flanking EGFP-IRES-Neo cassette (TFAP2C Human)
UseCRISPRTagsExpressionMutationrs6025024, hg38:20:56638660:TTT/-PromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISL1-donor
Plasmid#113820PurposeCRISPR donor plasmid to tag human transcription factor ISL1 with GFPDepositorInsertISL1 homology arms flanking EGFP-IRES-Neo cassette (ISL1 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDDIT3-donor
Plasmid#112300PurposeCRISPR donor plasmid to tag human transcription factor DDIT3 with GFPDepositorInsertDDIT3 homology arms flanking EGFP-IRES-Neo cassette (DDIT3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHOXB5-donor
Plasmid#113815PurposeCRISPR donor plasmid to tag human transcription factor HOXB5 with GFPDepositorInsertHOXB5 homology arms flanking EGFP-IRES-Neo cassette (HOXB5 Human)
UseCRISPRTagsExpressionMutationrs2288276, rs7406798, rs36049616, rs3087996PromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLOCK-donor
Plasmid#112310PurposeCRISPR donor plasmid to tag human transcription factor CLOCK with GFPDepositorInsertCLOCK homology arms flanking EGFP-IRES-Neo cassette (CLOCK Human)
UseCRISPRTagsExpressionMutationhg38:4:55435551:A>G, hg38:4:55435749:G>APromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF416-donor
Plasmid#112372PurposeCRISPR donor plasmid to tag human transcription factor ZNF416 with GFPDepositorInsertZNF416 homology arms flanking EGFP-IRES-Neo cassette (ZNF416 Human)
UseCRISPRTagsExpressionMutationhg38:19:57572882:A>G,hg38:19:57572932:C>G,h…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHF-donor
Plasmid#113817PurposeCRISPR donor plasmid to tag human transcription factor EHF with GFPDepositorInsertEHF homology arms flanking EGFP-IRES-Neo cassette (EHF Human)
UseCRISPRTagsExpressionMutationrs3818514, rs11032804, hg38:11:34659087:G>APromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCL6-donor
Plasmid#112389PurposeCRISPR donor plasmid to tag human transcription factor BCL6 with GFPDepositorInsertBCL6 homology arms flanking EGFP-IRES-Neo cassette (BCL6 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSRF-donor
Plasmid#112362PurposeCRISPR donor plasmid to tag human transcription factor SRF with GFPDepositorInsertSRF homology arms flanking EGFP-IRES-Neo cassette (SRF Human)
UseCRISPRTagsExpressionMutationhg38:6:43178470:C>A, hg38:6:43178514:G>APromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF766-donor
Plasmid#112352PurposeCRISPR donor plasmid to tag human transcription factor ZNF766 with GFPDepositorInsertZNF766 homology arms flanking EGFP-IRES-Neo cassette (ZNF766 Human)
UseCRISPRTagsExpressionMutationhg38:19:52290357:G>T, hg38:19:52290373:A>G,…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVEZF1-donor
Plasmid#112341PurposeCRISPR donor plasmid to tag human transcription factor VEZF1 with GFPDepositorInsertVEZF1 homology arms flanking EGFP-IRES-Neo cassette (VEZF1 Human)
UseCRISPRTagsExpressionMutationrs999907002, hg38:17:57975308:A>T, hg38:17:579…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pELK3-donor
Plasmid#112322PurposeCRISPR donor plasmid to tag human transcription factor ELK3 with GFPDepositorInsertELK3 homology arms flanking EGFP-IRES-Neo cassette (ELK3 Human)
UseCRISPRTagsExpressionMutationhg38:12:96266960:A>T, rs760335647, rs6538704PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUC2::GNSI (NrsR)
Plasmid#121537PurposeThis plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR
UseTagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …PromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS1212
Plasmid#63785PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection markerDepositorInserteGFP-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseGFP-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCY 3040-05
Plasmid#36220DepositorInsertLEU2
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEGFP-LEU2ExpressionMutationPromoterAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3040-04
Plasmid#36219DepositorInsertTRP1
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEGFP-TRP1ExpressionMutationPromoterAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBALU9
Plasmid#69324PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOPDepositorInsertgSL2-NLS-GFPint-FgF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNM3746
Plasmid#154109PurposeloxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'
UseCre/Lox; RmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNR4A1-donor
Plasmid#112295PurposeCRISPR donor plasmid to tag human transcription factor NR4A1 with GFPDepositorInsertNR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
UseCRISPRTagsExpressionMutationhg38:12:52058840:C>T, hg38:12:52058930:A>C,…PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAZ-donor
Plasmid#112359PurposeCRISPR donor plasmid to tag human transcription factor MAZ with GFPDepositorInsertMAZ homology arms flanking EGFP-IRES-Neo cassette (MAZ Human)
UseCRISPRTagsExpressionMutationhg38:16:29809765:G>APromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJUNB-donor
Plasmid#112313PurposeCRISPR donor plasmid to tag human transcription factor JUNB with GFPDepositorInsertJUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
UseCRISPRTagsExpressionMutationhg38:19:12792992:C>G, hg38:19:12793018:T>G,…PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHEY1-donor
Plasmid#112297PurposeCRISPR donor plasmid to tag human transcription factor HEY1 with GFPDepositorInsertHEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
UseCRISPRTagsExpressionMutationhg38:8:79765311:G>APromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDLX1-donor
Plasmid#112343PurposeCRISPR donor plasmid to tag human transcription factor DLX1 with GFPDepositorInsertDLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
UseCRISPRTagsExpressionMutationhg38:2:172087403:G>C,hg38:2:172087433:G>A,h…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pE2F5-donor
Plasmid#112351PurposeCRISPR donor plasmid to tag human transcription factor E2F5 with GFPDepositorInsertE2F5 homology arms flanking EGFP-IRES-Neo cassette (E2F5 Human)
UseCRISPRTagsExpressionMutationhg38:8:85213859:T>C, hg38:8:85213860:T>G, h…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF354B-donor
Plasmid#112309PurposeCRISPR donor plasmid to tag human transcription factor ZNF354B with GFPDepositorInsertZNF354B homology arms flanking EGFP-IRES-Neo cassette (ZNF354B Human)
UseCRISPRTagsExpressionMutationhg38:5:178884427:G>A, hg38:5:178883445:C>T,…PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL2316
Plasmid#137063PurposeloxP (empty) expression cassette: n-terminal GFPDepositorTypeEmpty backboneUseLeishmania floxed gene expressionTagsGFPExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2315
Plasmid#137055PurposeloxP (empty) expression cassette: c-terminal GFPDepositorTypeEmpty backboneUseLeishmania floxed gene expressionTagsGFPExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_219
Plasmid#164559PurposeLentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsFirst sgRNA cassette
Second sgRNA cassette
UseCRISPR and Lentiviral; Dual sgrnaTagsExpressionMammalianMutationPromoterHuman U6 and Mouse U6Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_220
Plasmid#164560PurposeLentiviral vector that encodes two sgRNA expression cassettes (one constitutive, one inducible via Flp). Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsFirst sgRNA cassette
Second sgRNA cassette
UseCRISPR and Lentiviral; Flp/frtTagsExpressionMammalianMutationPromoterHuman U6 and Mouse U6Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3
Plasmid#67276PurposeRMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PADepositorInsertsGFP (mWasabi)
hygro
UseTagsF3 and FrtExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTFE3-donor
Plasmid#112377PurposeCRISPR donor plasmid to tag human transcription factor TFE3 with GFPDepositorInsertTFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
UseCRISPRTagsExpressionMutationhg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLX-donor
Plasmid#112370PurposeCRISPR donor plasmid to tag human transcription factor MLX with GFPDepositorInsertMLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
UseCRISPRTagsExpressionMutationhg38:17:42570798:G>A, hg38:17:42570950:T>G,…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHRB-donor
Plasmid#112333PurposeCRISPR donor plasmid to tag human transcription factor THRB with GFPDepositorInsertTHRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF445-donor
Plasmid#112332PurposeCRISPR donor plasmid to tag human transcription factor ZNF445 with GFPDepositorInsertZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
UseCRISPRTagsExpressionMutationrs78585428, hg38:3:44446218:C>T, hg38:3:444466…PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF785-donor
Plasmid#112317PurposeCRISPR donor plasmid to tag human transcription factor ZNF785 with GFPDepositorInsertZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
UseCRISPRTagsExpressionMutationhg38:16:30582292:A>T, hg38:16:30582302:A>T,…PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGH50
Plasmid#107254PurposeVipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.DepositorInsertvipp1-gfp-fkbp12
UseSuicide vector for destined for double homologous…TagsGFP and FKBP12ExpressionBacterialMutationPromoternativeAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDT305
Plasmid#128801PurposeHuman codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backboneDepositorInsertdCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
UseCRISPR and LentiviralTagsExpressionMutationPromoterhUBC promoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only