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Showing: 221 - 240 of 613 results

  1. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

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  2. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162666-plasmid-map-sequence-id-321465

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  3. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162668-plasmid-map-sequence-id-321523

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  6. MZB131

    Plasmid
    #217839
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged AP3D1 adaptin appendage (920-1153) for pulldown assays
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    AP3D1 (AP3D1 Human)
    Use
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Deleted residues 1-919
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217839-plasmid-map-sequence-id-432995

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  7. pETDuet-TFIIF

    Plasmid
    #171082
    Purpose
    Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    RAP30 (GTF2F2 Human)
    RAP74 (GTF2F1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171082-plasmid-map-sequence-id-335551

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  10. UPLC1

    Plasmid
    #25550
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UPLC1 (ASAP3 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. MZB207

    Plasmid
    #217843
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to AP3D1 amphipathic helix (2-15) fused to mGreenLantern
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    mGreenLantern
    Use
    Tags
    10x His, AP3D1 (2-15), and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217843-plasmid-map-sequence-id-433002

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  15. MZB208

    Plasmid
    #217844
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to AP3M1 amphipathic helix (135-151) fused to mGreenLantern
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    mGreenLantern
    Use
    Tags
    10x His, AP3M1 (135-151), and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217844-plasmid-map-sequence-id-433004

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Showing: 221 - 240 of 613 results