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pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

Plasmid

#194687

Purpose

CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap entry vector

Plasmid

#188249

Purpose

Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pB6-Hipp11-DTA-Capecchi-for

Plasmid

#126649

Purpose

B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number

Depositor

Mario Capecchi

Type

Empty backbone

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40

Plasmid

#194690

Purpose

hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

AAV

Tags

mEGFP

Expression

Mutation

Promoter

hSyn1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40

Plasmid

#194691

Purpose

hSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

AAV

Tags

mEGFP

Expression

Mutation

Promoter

hSyn1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40

Plasmid

#194692

Purpose

hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

AAV

Tags

mEGFP

Expression

Mutation

Promoter

hSyn1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pITR-AAV-Rep2Cap9-GFP

Plasmid

#199744

Purpose

Expresses AAV2 Rep and AAV9 Cap, with GFP replacing a region of Cap, within AAV2 ITRs.

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV9 Cap w/ GFP insert

Use

AAV

Tags

Expression

Mammalian

Mutation

Replaced amino acids 356-736 in Cap with GFP

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET28C-SARS-CoV-2-Nucleocapsid

Plasmid

#204402

Purpose

E. coli expression of full-length SARS-CoV-2 nucleocapsid

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

SARS-CoV-2 nucleocapsid (N SARS-CoV-2)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

Plasmid

#194693

Purpose

CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

Promoter

CMV

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro_CMV_NES-Caprola_on-mEGFP

Plasmid

#194694

Purpose

CMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

Plasmid

#194695

Purpose

CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Pet15b-Endosomal Escape Control

Plasmid

#186552

Purpose

Negative Control confirming the need for a CPP peptide in mediating internalization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

6H-ETA-GFP

Use

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Pet15b-Internalization and Endosomal Escape

Plasmid

#186551

Purpose

Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-ETA-EGFP

Use

Tags

8xHis and EGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pB6-ROSA26-DTA-Capecchi-for

Plasmid

#125743

Purpose

B6J ROSA26 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number

Depositor

Mario Capecchi

Type

Empty backbone

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

Plasmid

#194682

Purpose

CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

Plasmid

#194684

Purpose

CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

Plasmid

#194688

Purpose

hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Use

AAV

Tags

mEGFP

Expression

Mutation

Promoter

hSyn1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP C1-Eps8 ∆cap/∆bund

Plasmid

#74891

Purpose

mammalian expression of the capping and bundling activity mutant Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

V689D, L693D, L757A, K759A

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
p270 pCMV-CRE-M-AR(LNCaP)

Plasmid

#12496

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

CRE-M AR (AR Human)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Thr 868 Ala in AR-LBD

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pOPINK-CAPN15 (N)ZF1-3 (1-173)

Plasmid

#235646

Purpose

Bacterial expression of human CAPN15 (N)ZF1-3 (1-173)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 (N)ZF1-3 (CAPN15 Human)

Use

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 1-173 only

Promoter

T7

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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