Addgene

Showing: 221 - 240 of 9506 results

Results per page:

Sort results by:

Human USP47

Plasmid

#217624

Purpose

Mammalian expression of USP47

Depositor

Ethan Lee

Article

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

USP47 (USP47 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
tet-pLKO shGATA6 puro

Plasmid

#72615

Purpose

Expresses an inducible short hairpin targeting human GATA6 sequence

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

short hairpin GATA binding protein 6

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

TRE Tight

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Luciferase-pcw107-V5

Plasmid

#64649

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

PGK

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
BtuF

Plasmid

#92209

Purpose

Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter

Depositor

Douglas Rees

Article

Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99

Insert

BtuF (btuF )

Use

Tags

6x His Tag and PelB Signal Sequence

Expression

Bacterial

Mutation

BtuF signal sequence removed and uses the PelB si…

Promoter

T7 Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_PGK_LTB4R

Plasmid

#208087

Purpose

Mammalian expression of the wild-type human LTB4 receptor LTB4R.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R (LTB4R Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

none

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Luciferase-pcw107

Plasmid

#64648

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)

Plasmid

#171375

Purpose

For the mammalian expression of rat Gαi3 internally tagged with YFP

Depositor

Mikel Garcia-Marcos

Article

Marivin et al Sci Signal. 2016 Apr 12;9(423):ra37. doi

Insert

Gai3-YFP

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HcRed-pcw107-V5

Plasmid

#64647

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

PGK

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
HcRed-pcw107

Plasmid

#64646

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only
Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

Plasmid

#52092

Purpose

Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

Depositor

Dario Vignali

Article

Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

Inserts

CD3 delta (Cd3d Mouse)
CDC gamma (Cd3g Mouse)
CD3 epsilon (Cd3e Mouse)
CD3 zeta (Cd247 Mouse)

Use

Retroviral

Tags

F2A, IRES-EGFP, P2A, and T2A

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

Plasmid

#52093

Purpose

Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

Depositor

Dario Vignali

Article

Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

Inserts

CD3 delta (Cd3d Mouse)
CDC gamma (Cd3g Mouse)
CD3 epsilon (Cd3e Mouse)
CD3 zeta (Cd247 Mouse)

Use

Retroviral

Tags

F2A, IRES-EGFP, P2A, and T2A

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Human Myc-XIAP

Plasmid

#217627

Purpose

Mammalian expression of Myc tagged XIAP

Depositor

Ethan Lee

Article

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

XIAP (XIAP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MO70-N-Flag-hCRACR2A-isoform-a

Plasmid

#79592

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
p-mCherry-C1-hCRACR2A-isoform-a

Plasmid

#79593

Purpose

Transient expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pLV_PGK_LTB4R(T308A)

Plasmid

#208088

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the T308A mutation.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R(T308A) (LTB4R Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T308A

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_PGK_LTB4R(S310A)

Plasmid

#208089

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the S310A mutation.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R(S310A) (LTB4R Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S310A

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_PGK_LTB4R(T308A,S310A)

Plasmid

#208090

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R(T308A,S310A) (LTB4R Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T308A, S310A

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_PGK_3xHA-LTB4R

Plasmid

#208091

Purpose

Mammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

3xHA-LTB4R (LTB4R Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

none

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPICZ-pre-Ost1-pro-alphaf-Btl2

Plasmid

#117665

Purpose

Study secretion efficiency of Btl2

Depositor

Benjamin Glick, Pau Ferrer

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-pro-alphaf-Btl2

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the lipase Btl2 together wit…

Promoter

pAOX1

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CROPseq-Guide-Puro-mCherry2

Plasmid

#219679

Purpose

CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

Depositor

Christoph Bock

Article

Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge