We narrowed to 5,545 results for: donor

1. insulator_seq_UENU_donor

   Plasmid

   #221459

   Purpose

   Vector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.

   Depositor

   Maria Cristina Gambetta

   Article

   Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

   Insert

   3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR
   

   Use

   Synthetic Biology

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCgVchDonor

   Plasmid

   #203817

   Purpose

   DNA donor for transposition, shuttle vector for Corynebacterium glutamicum / E. coli

   Depositor

   Sheng Yang

   Article

   Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

   Insert

   Mini-Tn, Spec cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Donor_YTHDF2_KO_Neo

   Plasmid

   #186672

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Neomycin (YTHDF2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Donor_ANKRD1_KO_Hygro

   Plasmid

   #186668

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO Hygromycin (ANKRD1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Donor_C_FH_VIR

   Plasmid

   #186660

   Purpose

   Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Donor_N_FH_Shu

   Plasmid

   #186655

   Purpose

   Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Donor_N_FH_Piwi

   Plasmid

   #186651

   Purpose

   Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Donor_C_FH_Nbr

   Plasmid

   #186662

   Purpose

   Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor_N_FH_GLE

   Plasmid

   #186657

   Purpose

   Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bxb1Donor_G315T_ADRB2_LucReporter

    Plasmid

    #129793

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    G315T

    Promoter

    N/A

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bxb1Donor_W99A_ADRB2_LucReporter

    Plasmid

    #129789

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    W99A

    Promoter

    N/A

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bxb1Donor_N51L_ADRB2_LucReporter

    Plasmid

    #129790

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    N51L

    Promoter

    N/A

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pReplace_donor_mcherry

    Plasmid

    #149345

    Purpose

    Donor vector for the replacement of reporter BFP by mcherry

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    mcherry
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AL62_pDonor.DMD.TS

    Plasmid

    #100287

    Purpose

    DMD intron 43-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    EGFP expression unit (DMD Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. AX44_pS.Donor.S1

    Plasmid

    #100289

    Purpose

    AAVS1-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Factor IX donor

    Plasmid

    #182141

    Purpose

    Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR
    

    Expression

    Mammalian

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pScaffold-H1 donor

    Plasmid

    #118152

    Purpose

    PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDonor_Tn[himar1C9]

    Plasmid

    #213906

    Purpose

    Mariner transposon with ATc-inducible himar1C9 transposase

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with cam resistance and ATc-inducible himar1C9 transposase
    

    Expression

    Bacterial

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL1119 (pDonor)

    Plasmid

    #160732

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFETCh_Donor (EMM0021)

    Plasmid

    #63934

    Purpose

    3X Flag with P2A-Neo for 3' tagging of endogenous proteins

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Glycine-Serine-3XFlag-P2A-NeoR

    Expression

    Mammalian

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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