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pMLS272

Plasmid

#73728

Purpose

SapTrap syntron-embedded FLP-on C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

syntron embedded C-terminal FLP-on

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS281

Plasmid

#73732

Purpose

SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

syntron embedded N-terminal FLP-on

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pJB94

Plasmid

#208752

Purpose

Theophylline-based control of repA on a C. difficile plasmid for use in allelic exchange

Depositor

Joseph Sorg

Article

Brehm et al Anaerobe. 2024 Apr 29;88:102858. doi: 10

Insert

sacB (sacB )

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS134

Plasmid

#73714

Purpose

SapTrap destination vector for building sgRNA expression vectors.

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

pU6::sgRNA

Use

CRISPR

Expression

Worm

Mutation

SapI insertion site

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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peZinCh-NB

Plasmid

#24173

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eZinCh-NB

Expression

Mammalian

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pGFP-HBx NESM

Plasmid

#24932

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Tags

EGFP and HA

Expression

Mammalian

Mutation

L98A,G99A,L100A

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pCFJ676 - MCS(ttTi4348, I, unc-18(+))

Plasmid

#34872

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4348 targeting

Use

Worm targeting

Available Since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCFJ326 - operon for germline expression

Plasmid

#34875

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

tbb-2 gld-1 operon

Use

Worm targeting

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS268

Plasmid

#73726

Purpose

SapTrap SL2 C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

SL2 (gdp-2/gdp-3 intergenic region)

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ448 - pDESTR4-R3(ttTi4348, I, unc-18)

Plasmid

#34865

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4348 targeting

Use

Worm targeting

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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peZinCh-1

Plasmid

#24172

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eZinCh-1

Expression

Mammalian

Mutation

Cerulean and Citrine both contain mutations Y39H …

Promoter

CMV

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMLS285

Plasmid

#73734

Purpose

SapTrap egl-13 NLS connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

egl-13 NLS

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCZ47

Plasmid

#128414

Purpose

vab-1 rescuing minigene

Depositor

Andrew Chisholm

Article

George et al Cell. 1998 Mar 6;92(5):633-43.

Insert

vab-1 (vab-1 Nematode)

Use

Unspecified

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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smtn-b

Plasmid

#78710

Purpose

Encodes zebrafish smtn-b

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

smtn-b (smtnb Zebrafish)

Expression

Bacterial

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-PTEN

Plasmid

#28298

Depositor

Maria-Magdalena Georgescu

Article

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pAF018 p3E-2A-mCerulean-CAAX

Plasmid

#195975

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann

Article

Kirchgeorg et al Dev Dyn. 2018 Oct;247(10):1146-1159. doi

Insert

2A-mCerulean-CAAX (membrane)

Use

Other

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ421 - Pmyo-2::GFP::H2B (pharynx)

Plasmid

#34876

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

Pmyo-2 GFP H2B tbb-2 UTR

Use

Worm targeting

Mutation

S65C

Promoter

myo-2

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ420 - Peft-3::GFP::H2B (ubiquitous)

Plasmid

#34877

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

Peft-3 GFP H2B tbb-2 UTR

Use

Worm targeting

Mutation

S65C

Promoter

eef-1A.1 (eft-3)

Available Since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCFJ151 - ttTi5605_MCS

Plasmid

#19330

Depositor

Erik Jorgensen

Article

Christian et al

Insert

ttTi5605 targeting region

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pCFJ150 - pDESTttTi5605[R4-R3]

Plasmid

#19329

Depositor

Erik Jorgensen

Article

Christian et al

Insert

pDESTttTi5605[R4-R3]

Expression

Worm

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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