We narrowed to 7,345 results for: Lif

1. pcDNA3.1-TEK-V5/HIS

   Plasmid

   #205611

   Purpose

   Expression of human TEK receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TEK receptor tyrosine kinase (TEK Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKC-4.06

   Plasmid

   #112084

   Purpose

   NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)

   Depositor

   James Inglese

   Article

   Baird et al Elife. 2018 Mar 12;7. pii: 33178. doi: 1

   Insert

   3XFLAG firefly luciferase-beta-globin 39PTC (HBB Photinus pyralis, Mouse, Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SEO-CaMKII-TVA-P2A-N2cG

   Plasmid

   #172363

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinase

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-shQKI-3373

   Plasmid

   #115456

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shQKI-3373 (QKI Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Venus-Venus-FLARE-Cameleon

   Plasmid

   #123351

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-Venus-FLARE-Cameleon
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMIA3-Pansio

   Plasmid

   #214445

   Purpose

   Expresses eSpCas9 and gRNA targeting Pansio-1 GSH

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   eSpCas9-P2A-mRuby2-P2A-tp53dn
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

   Plasmid

   #127353

   Purpose

   gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Expression

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-guide-puro mMyh9 - 2

   Plasmid

   #198492

   Purpose

   lentiviral stable expression of mMyh9 gRNA 2

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mMyh9 (Myh9 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-guide-puro mMyh9 - 1

   Plasmid

   #198491

   Purpose

   lentiviral stable expression of mMyh9 gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mMyh9 (Myh9 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAW-NbVHH05-mCherry

    Plasmid

    #171572

    Purpose

    Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-mCherry (UBE2J1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTT3 Unc5A-FLAG

    Plasmid

    #72194

    Purpose

    Expresses full-length Unc5a with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5a (Unc5a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTP261

    Plasmid

    #104166

    Purpose

    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    

    Use

    Tags

    14x Histidine tag and NEDD8 from Brachypodium dis…

    Expression

    Bacterial

    Mutation

    1x Cysteine located at bps 493-495 in full sequen…

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTT3 Unc5B-FLAG

    Plasmid

    #72195

    Purpose

    Expresses full-length Unc5b with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5b (Unc5b Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGY577

    Plasmid

    #166663

    Purpose

    Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cre: E340A, D341A

    Promoter

    CMV

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. SOX9-T2A-H2B-EGFP repair template

    Plasmid

    #167972

    Purpose

    SOX9 reporter repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SRY-Box Transcription Factor 9 (SOX9 Human)
    

    Use

    CRISPR; Donor template

    Tags

    H2B-EGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

    Plasmid

    #127355

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and mVenus

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pES002 MBP-GSK3β_S9A-HA-His

    Plasmid

    #196184

    Purpose

    Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Tags

    HA, His6, and MBP

    Expression

    Bacterial

    Mutation

    changed Serine 9 to Alanine

    Promoter

    Tac

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

    Plasmid

    #185676

    Purpose

    SpCas9 and gRNA targeting the C-terminus of HsIRE1

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 gRNA (ERN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

19. pBbA5a-sufCDSUB

    Plasmid

    #195055

    Purpose

    Expresses SUF operon from B. subtilis from IPTG-inducible promoter

    Depositor

    Greg Bokinsky

    Article

    D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

    Insert

    sufCDSUB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCB306-GFP-Rab8A

    Plasmid

    #198470

    Purpose

    lentiviral expression of GFP-Rab8A

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    GFP-Rab8A (RAB8A Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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