We narrowed to 4,947 results for: mcs

1. pTrc99S-ssDsbA-TTlight-4xDQNAT

   Plasmid

   #128402

   Purpose

   Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin light chain domain
   

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Inactivating E234A mutation

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA4.TO-ORF62-2xCSTREP

   Plasmid

   #136222

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF62

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF62
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA4.TO-ORF6-2xCSTREP

   Plasmid

   #136170

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF6

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF6
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-hPCM2w/b delta6

   Plasmid

   #47588

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA2w/b delta6 (ATP2B2 Human)
   

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Mutation

   6 C-terminal AA deleted (needed for PDZ binding)

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA4.TO-ORF74-2xNSTREP

   Plasmid

   #136233

   Purpose

   Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF74

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF74
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-EZH2-T487A mutant

   Plasmid

   #28061

   Depositor

   Mien-Chie Hung

   Article

   Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

   Insert

   EZH2 (EZH2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T487A

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pODN-mNG-ECT2

   Plasmid

   #183835

   Purpose

   Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 homology arms with mNeonGreen-linker (ECT2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV superYFP-AURKA-mTurq2

   Plasmid

   #157772

   Purpose

   Expression of AuroraA kinase biosensor under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   mTurquoise2 and superYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA4.TO-ORF68-2xCSTREP

   Plasmid

   #136229

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF68

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF68
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3_GCGR-NTEV-TCS-GV-2xHA

    Plasmid

    #194374

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    GCGR-NTEV-TCS-GV-2xHA (GCGR Human, Synthetic)
    

    Tags

    2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA4.TO-ORF61-2xCSTREP

    Plasmid

    #136221

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF61

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF61
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pINDUCER21-puro_TAOK2

    Plasmid

    #172979

    Purpose

    doxycycline-inducible expression of TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    TAOK2 (TAOK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA4.TO-K1-2xCSTREP

    Plasmid

    #136148

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K1

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-1 IntS6 AA 1035-1284

    Plasmid

    #196904

    Purpose

    Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 6 (IntS6 Fly)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA4.TO-ORF33-2xCSTREP

    Plasmid

    #136194

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF33

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF33
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA4.TO-ORF60-2xCSTREP

    Plasmid

    #136220

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF60

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF60
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA4.TO-ORF38-2xCSTREP

    Plasmid

    #136199

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF38

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF38
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

    Plasmid

    #198054

    Purpose

    All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning

    Depositor

    Lacra Bintu

    Article

    Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.

    Inserts

    rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
    3xFLAG-insert
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    TRE3G and pEF

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA4.TO-ORF63-2xCSTREP

    Plasmid

    #136223

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF63

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF63
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA4.TO-K8a-2xCSTREP

    Plasmid

    #136158

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8a

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K8a
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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