We narrowed to 31,889 results for: Eng

1. WT1-2A-eGFP PGK PuroR Donor Plasmid

   Plasmid

   #82333

   Purpose

   WT1 targeting donor

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Inserts

   WT1 5' Arm (WT1 Human)
   WT1 3' Arm (WT1 )
   

   Use

   CRISPR and TALEN

   Tags

   2A-eGFP

   Expression

   Mammalian

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

   Plasmid

   #208290

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations and V106W in TadA and nSpCas9(D10…

   Promoter

   CMV and T7

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)

   Plasmid

   #208292

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA inlaid into nSpCas9(D10A)

   Promoter

   CMV and T7

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ad:2CMV_A2R2-N25Ctr

   Plasmid

   #122243

   Purpose

   Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

   Depositor

   Corey Neu

   Article

   Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

   Inserts

   C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
   Actinin alpha 2 (Actn2 Mouse)
   

   Use

   Adenoviral

   Tags

   Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

   Expression

   Mammalian

   Mutation

   deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…

   Promoter

   CMV

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CYLBL_Neo_CAGdCas9-KRAB

   Plasmid

   #220439

   Purpose

   CAG-dCas9-KRAB knock-in using TALEN at CLYBL locus

   Depositor

   Yin Shen

   Article

   Yang et al Nat Genet. 2023 Oct;55(10):1735-1744. do

   Insert

   dCas9
   

   Use

   CRISPR and TALEN

   Tags

   KRAB

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6-sgR26-eCas9-T2A-BlastR

   Plasmid

   #172493

   Purpose

   For CRISPR-assisted HDR, Rosa26 locus

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Insert

   eCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

   Plasmid

   #197505

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

   Promoter

   CMV and T7

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-crRNA(SOD1)

   Plasmid

   #109321

   Purpose

   AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1

   Depositor

   Feng Zhang

   Article

   Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

   Insert

   mCherry-KASH (SOD1 Human)
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MKC86_HBA1(tEPOR-2A-YFP)

   Plasmid

   #232405

   Purpose

   AAV production plasmid for tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at HBA1 locus using HBA1-sg4 gRNA. HBA1 UTRs flank full tEPOR-2A-YFP cassette. Homology arms mediate full HBA1 replacement.

   Depositor

   Kyle Cromer, Matthew Porteus

   Article

   Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

   Inserts

   Truncated Erythropoietin Receptor (EPOR Human)
   Hemoglobin Subunit Alpha 1 (HBA1 Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN

    Plasmid

    #172435

    Purpose

    Lentivirus, expresses Cre recombinase, mouse PVR (CD155), and mScarlet-SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    Cre
    PVR
    mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
    

    Use

    Lentiviral

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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