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pEY83

Plasmid

#191078

Purpose

flp-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-8 (flp-8 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)

Plasmid

#191176

Purpose

Mammalian expression of EK0209 with a second stop codon.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP (double-stop)

Expression

Mammalian

Mutation

additional stop codon

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0546 SFFV-mCherry-SGc(GAPDH.s1-dblstop)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191178

Purpose

Mammalian expression of EK0493 with second stop codon.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP (double-stop)

Expression

Mammalian

Mutation

additional stop codon

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)

Plasmid

#191166

Purpose

Mammalian expression of a EGFP mutant N213D derived from EK0379.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

EGFP(N213D)

Expression

Mammalian

Mutation

c.637A>G(N213D; CCA>CCG)

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

Plasmid

#191148

Purpose

AAV expression of a Cre reporter with EYFP output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

DIO-EYFP

Use

AAV

Expression

Mammalian

Mutation

WT

Promoter

hEF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0362 SFFV-mCherry-SG(Bdnf.s1L36+54@202)SG-EGFP (FLP-IN)

Plasmid

#191153

Purpose

Mammalian expression of a 90 bp (36+54) long sensor for murine Bdnf 3' UTR where the trigger sequence is split

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1L36+54:EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_dPC_2xChromo

Plasmid

#179398

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

dPC_2xChromo

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-SpRYgest_targetplasmid (KAC833)

Plasmid

#181748

Purpose

pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Type

Empty backbone

Use

Substrate plasmid

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBI101.1/ProGOXL6:GUS

Plasmid

#175562

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL6

Tags

GUS

Expression

Plant

Promoter

GOXL6

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL5:GUS

Plasmid

#175561

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL5

Tags

GUS

Expression

Plant

Promoter

GOXL5

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL4:GUS

Plasmid

#175560

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL4

Tags

GUS

Expression

Plant

Promoter

GOXL4

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL3:GUS

Plasmid

#175559

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL3

Tags

GUS

Expression

Plant

Promoter

GOXL3

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL2:GUS

Plasmid

#175558

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL2

Tags

GUS

Expression

Plant

Promoter

GOXL2

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CaMKII-GCaMP6f-4x128-mAGNET

Plasmid

#166023

Purpose

Expresses GCaMP6f in CaMKII+ cells with low miRNA-128 expression

Depositor

Xue Han

Article

Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

Insert

GCaMP6f with 4 miR-128 binding sites

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSWD95Y14D

Plasmid

#159098

Purpose

CckA FRET Sensor with cyclic-di-GMP point mutant

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3

Expression

Bacterial

Mutation

Mutated Y514 to D514

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSWD95D293A

Plasmid

#159099

Purpose

CckA FRET Sensor with DITE motif mutant

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3

Expression

Bacterial

Mutation

CckA D293 mutated to A293 and V199I on mRuby3 (P…

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL710

Plasmid

#140380

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 14BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-14BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL702

Plasmid

#140372

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 0BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-0BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL706

Plasmid

#140376

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 6BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-6BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL707

Plasmid

#140377

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 8BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-8BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL708

Plasmid

#140378

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 10BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-10BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL709

Plasmid

#140379

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 12BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-12BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJB03

Plasmid

#111961

Purpose

Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Inserts

mEGFP
eDHFR

Tags

DHFR

Expression

Bacterial

Mutation

eDHFR;residues 2-159 and enhanced GFP with monome…

Promoter

Tac

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1400

Plasmid

#78190

Purpose

Ce hsp-16.1 expression in worms

Depositor

Junho Lee

Article

Lee et al J Biol Chem. 2013 Jan 18;288(3):1582-9.

Insert

hsp-16.1 (hsp-16.1 Nematode)

Expression

Worm

Promoter

hsp-16.1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pCRII HSPB8

Plasmid

#61035

Purpose

Probe for HSPB8 for in situ hybridization in zebrafish

Depositor

Lara Hutson

Article

Marvin et al Dev Dyn. 2008 Feb;237(2):454-63.

Insert

HSPB8 (hspb8 Zebrafish)

Expression

Bacterial

Available Since

Dec. 16, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM29i2

Plasmid

#59607

Purpose

Expression of shRNA against human ADAM29

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM29 (ADAM29 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM7i2

Plasmid

#59603

Purpose

Expression of shRNA against human ADAM7

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM7 (ADAM7 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-DRAM1i2

Plasmid

#59597

Purpose

Expression of shRNA against human DRAM1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

DRAM1 (DRAM1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-DRAM1i1

Plasmid

#59596

Purpose

Expression of shRNA against human DRAM1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

DRAM1 (DRAM1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
TAL3085

Plasmid

#41259

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-ghsra-Right (ghsra Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only