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Showing: 4801 - 4840 of 8321 results

  1. pIRES-Neo-HA-cdc25C5

    Plasmid
    #40967
    Depositor
    Olivier Gavet
    Insert
    cdc25C5 (CDC25C Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. BC-GPRC

    Plasmid
    #44199
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    C-terminal fragment of GPR56 starting at amino a…
    Promoter
    LTR
    Available since
    May 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44199-plasmid-map-sequence-id-60058

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  3. pTF260 (pFA6a-PSc-6xGly-KanMX)

    Plasmid
    #44086
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44086-plasmid-map-sequence-id-61663

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  4. pJK142

    Plasmid
    #73317
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6, Pas2p, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene. Encodes a glycine-204 to serine m…
    Promoter
    AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73317-plasmid-map-sequence-id-140432

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  5. pUC57-sgDedd-1

    Plasmid
    #74435
    Purpose
    sgRNA expression vector for mouse Dedd gene targeting intron 5
    Depositor
    Petra Arck
    Insert
    Dedd (Dedd Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pPyCAG-Nanog(K137A)-IP

    Plasmid
    #62996
    Purpose
    Expresses Mouse Nanog K137A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K137A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Lysine 137 (138 AA in Mouse Protein) to A…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62996-plasmid-map-sequence-id-104598

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  7. pPyCAG-Nanog(Y119A)-IP

    Plasmid
    #62991
    Purpose
    Expresses Mouse Nanog Y119A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y119A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 119 (120 AA in Mouse Protein) to…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62991-plasmid-map-sequence-id-104440

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  8. pIRES-GFP-hFJX1-myc

    Plasmid
    #46928
    Purpose
    coexpression of FJX1-myc and GFP in mammalian cells
    Depositor
    Natasha Deane, R.D. Beauchamp
    Insert
    FJX1 (FJX1 Human)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 13, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. ACBD4

    Plasmid
    #39009
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACBD4 (ACBD4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pUASp YFP Rholike CA

    Plasmid
    #52241
    Purpose
    Drosophila expression of YFP-Rholike constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Rholike (RhoL Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q69L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. 4LG9

    Plasmid
    #60096
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TBL1XR1:APC048-A06:C27585 (TBL1XR1 Human, TBL1XR1:APC048-A06:C27585)
    Use
    Baculovirus expression
    Tags
    6xHis
    Expression
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pTF265 (pFA6a-PSc-6xGly-12xHis-His3MX)

    Plasmid
    #44063
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44063-plasmid-map-sequence-id-61668

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  14. pHygroMod-Foxd3-3xFlag

    Plasmid
    #37274
    Depositor
    Patricia Labosky
    Insert
    Foxd3 (Foxd3 Mouse)
    Use
    Mouse Targeting; Insertion vector for rmce
    Tags
    Flag tag on Foxd3
    Expression
    Mutation
    Promoter
    Available since
    July 31, 2012
    Availability
    Academic Institutions and Nonprofits only

  15. TDRD5

    Plasmid
    #32869
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD5 (TDRD5 Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pBOBI-hPLD3-flag

    Plasmid
    #222640
    Purpose
    lentiviral vector for full length human PLD3 expression (with flag tag)
    Depositor
    David Nemazee
    Insert
    PLD3 (PLD3 Human)
    Use
    Lentiviral
    Tags
    flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    222640-plasmid-map-sequence-id-472427

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  17. pSecUAG_UTu1D

    Plasmid
    #233867
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant
    Depositor
    Natalie Krahn, Dieter Söll
    Inserts
    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG) and …
    Promoter
    EM7, araBAD, and native As SelD
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233867-plasmid-map-sequence-id-471093

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  18. pQdCas12a.sggfp(A)

    Plasmid
    #236038
    Purpose
    The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236038-plasmid-map-sequence-id-469933

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  19. pQdCas12a.sggfp(B)

    Plasmid
    #236040
    Purpose
    The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236040-plasmid-map-sequence-id-469924

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  20. Ec dnaN-pHK/P

    Plasmid
    #237239
    Purpose
    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)
    Depositor
    Michael O'Donnell
    Insert
    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    Use
    Tags
    his-10+PKA kinase motif+PreScission Protease clea…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237239-plasmid-map-sequence-id-469256

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  21. pRDA_852

    Plasmid
    #185527
    Purpose
    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    185527-plasmid-map-sequence-id-366261

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  22. 1996_pCCLsin.PPT.hPGK.EGFP-mCEP83.PRE

    Plasmid
    #231805
    Purpose
    Label the mother centrosome-specific distal appendage protein CEP83 by electroporating an mRNA encoding it as well as an eGFP fusion protein.
    Depositor
    Annette Oxenius
    Insert
    eGFP-mCEP83
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231805-plasmid-map-sequence-id-460689

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  24. pX330-MM002-ZFP143-C-terminal-sgRNA

    Plasmid
    #212706
    Purpose
    Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus
    Depositor
    Elzo de Wit
    Insert
    ZFP143 C-terminal sgRNA (Zfp143 Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    212706-plasmid-map-sequence-id-438797

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  25. pENTR/hBIG1

    Plasmid
    #226251
    Purpose
    Entry plasmid of hBIG1 for Gateway system
    Depositor
    Hye-Won Shin
    Insert
    BIG1 (ARFGEF1 Human)
    Use
    Entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226251-plasmid-map-sequence-id-454235

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  26. pENTR/HA-hBIG2

    Plasmid
    #226252
    Purpose
    Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system
    Depositor
    Hye-Won Shin
    Insert
    BIG2 (ARFGEF2 Human)
    Use
    Entry vector
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226252-plasmid-map-sequence-id-454237

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  27. pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA

    Plasmid
    #216665
    Purpose
    Expresses Gal4 inducible BFP, with hygromycin resistance
    Depositor
    Leonardo Morsut
    Inserts
    BFP
    HygromycinR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216665-plasmid-map-sequence-id-432189

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  29. pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

    Plasmid
    #225432
    Purpose
    Transcriptional unit of BeYDV Rep/RepA (C1:C2)
    Depositor
    Diego Orzaez
    Insert
    pNOS:Rep/RepA:tNOS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225432-plasmid-map-sequence-id-447739

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  30. pEN_TT DNM1L-V5

    Plasmid
    #220359
    Purpose
    Gateway entry vector for an inducible V5-tagged DNM1L
    Depositor
    Kevin Janes
    Insert
    DNM1L (DNM1L Human)
    Use
    Entry vector for gateway cloning
    Tags
    V5
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220359-plasmid-map-sequence-id-447351

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  31. pET-UBE3A iso 2 N terminus

    Plasmid
    #185643
    Purpose
    expression of His-tagged UBE3A isoform 2 1-87 in bacterial cells
    Depositor
    Irina Bezsonova
    Insert
    ubiquitin-protein ligase E3A (UBE3A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Sept. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    185643-plasmid-map-sequence-id-367329

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  32. pCDNA3.SLC17A2-flag

    Plasmid
    #224419
    Purpose
    Expresses SLC17A2 in mammalian cells
    Depositor
    Jonathan Long
    Insert
    Solute carrier family 17 (sodium phosphate), member 2 (SLC17A2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    224419-plasmid-map-sequence-id-444193

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  33. CD55-Delta3

    Plasmid
    #220872
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-3
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-3, short consensus repeat (SCR) domains 1, …
    Promoter
    CMV
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220872-plasmid-map-sequence-id-443043

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  34. CD55-Delta4

    Plasmid
    #220873
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-4
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-4, short consensus repeat (SCR) domains 1, …
    Promoter
    CMV
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220873-plasmid-map-sequence-id-443042

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  35. CD55-Delta1

    Plasmid
    #220870
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-1
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-1, the first short consensus repeat (SCR) d…
    Promoter
    CMV
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220870-plasmid-map-sequence-id-443041

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  36. CD55-Delta2

    Plasmid
    #220871
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-2
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-2, short consensus repeat (SCR) domains 1 a…
    Promoter
    CMV
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220871-plasmid-map-sequence-id-443038

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  37. hs-cre::EBD, UAS-dBrainbow attB

    Plasmid
    #220326
    Purpose
    expresses a heat-shock inducible cre::EBD fusion protein and UAS-dBrainbow
    Depositor
    Erdem Bangi
    Inserts
    Cre::EBD (with intron)
    dBrainbow
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    UAS and hs
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    220326-plasmid-map-sequence-id-441806

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  38. pCMV-PE2-P2A-PuroR

    Plasmid
    #196962
    Purpose
    Expresses PE2 with puromycin resistance
    Depositor
    Leopold Parts
    Insert
    PE2-P2A-Puro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    196962-plasmid-map-sequence-id-440213

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  39. pCDF-B-pC

    Plasmid
    #213495
    Purpose
    Encodes Node B of the three-node Turing circuit
    Depositor
    Mark Isalan
    Inserts
    cinI (cinI )
    lacI (lacI )
    lambda cI (lambdap50 )
    sfGFP
    Use
    Synthetic Biology
    Tags
    ASV tag and LVA tag
    Expression
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    213495-plasmid-map-sequence-id-430628

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  40. pYES2/NTA/Leu-LegA7Δank

    Plasmid
    #216519
    Purpose
    Expression of LegA7 ΔAnk
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔAnk
    Use
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 331-454
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216519-plasmid-map-sequence-id-430063

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Showing: 4801 - 4840 of 8321 results