We narrowed to 21,628 results for: ESC

1. pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)

   Plasmid

   #173458

   Purpose

   C-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mCherry-sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-MtrxRFP2

   Plasmid

   #172323

   Purpose

   fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

   Depositor

   Huiwang Ai

   Article

   Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

   Insert

   human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET19b-rDAD2 cPGFP(N180)

   Plasmid

   #166001

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rDAD2 cPGFP(N180)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LSSmOrange- V49A (please see depositor comments)

   Promoter

   T7 promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-deLACCO

   Plasmid

   #167945

   Purpose

   Fluorescent biosensor control for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   deLACCO
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SEPLuc-E2A-cyOFP1

   Plasmid

   #141084

   Purpose

   precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   SEPLuc-E2A-cyOFP1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. P302-FBP_1

   Plasmid

   #139692

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   p35S_Short_TMV 5':AsNpga:tAtug7, AtUbi10:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. P313-FBP_8

   Plasmid

   #139699

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing H3H

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp, pAtuMas:Nn Luz:trbcSE9
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBW-m4046911

   Plasmid

   #145695

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 helicase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046838

   Plasmid

   #145696

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGBW-m4046840

    Plasmid

    #145700

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp10

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046894

    Plasmid

    #145688

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp10

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGBW-m4046732

    Plasmid

    #145677

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGBW-m4046969

    Plasmid

    #145678

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4046975

    Plasmid

    #145679

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGBW-m4046913

    Plasmid

    #145680

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046872

    Plasmid

    #145667

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7b

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046858

    Plasmid

    #145658

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046730

    Plasmid

    #145659

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046968

    Plasmid

    #145652

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp10

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046936

    Plasmid

    #145656

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge