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We narrowed to 14,439 results for: eng

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Showing: 2401 - 2420 of 14439 results

  1. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65779-plasmid-map-sequence-id-112660

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  4. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    175570-plasmid-map-sequence-id-344992

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  10. pcDNA3.1-dCas9-dMQ1-EGFP

    Plasmid
    #89637
    Purpose
    Targeted CpG methylation
    Depositor
    Margaret Goodell
    Publication
    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    site-specific DNA-methyltransferase SssI
    Use
    CRISPR
    Tags
    3*FLAG, 6*His, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    C141S, TGC-->TCC; S317A, AGC-->GCC
    Promoter
    CMV
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89637-plasmid-map-sequence-id-177705

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  15. pCS6-YFP-SMASh(TI)

    Plasmid
    #111501
    Purpose
    Expresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Michael Lin
    Publication
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP-SMASh(TI)
    Tags
    HA epitope tag and SMASh tag
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, S122R, a…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111501-plasmid-map-sequence-id-214268

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  17. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Publication
    Newman et al bioRxiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170510-plasmid-map-sequence-id-343694

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Showing: 2401 - 2420 of 14439 results