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pNLF1-N_SEC23B:1-767

Plasmid

#210988

Purpose

Mammalian expression of full-length human SEC23B. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SEC23B:M1-C767 (SEC23B Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_FBXW5:1-566

Plasmid

#210981

Purpose

Mammalian expression of full-length human FBXW5. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW5:M1-R566 (FBXW5 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_PWP1:1-501

Plasmid

#210975

Purpose

Mammalian expression of full-length human PWP1. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PWP1:M1-S501 (PWP1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

E165D F448L G449R not annotated variants

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_MAGEA3:1-314

Plasmid

#210977

Purpose

Mammalian expression of full-length human MAGEA3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

MAGEA3:M1-E314 (MAGEA3 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_RFWD3:1-774

Plasmid

#210955

Purpose

Mammalian expression of full-length human RFWD3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RFWD3:M1-E774 (RFWD3 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_WDR55:1-383

Plasmid

#210957

Purpose

Mammalian expression of full-length human WDR55. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR55:M1-D383 (WDR55 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

C151R natural variant (VAR_037057)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_WDR12:1-423

Plasmid

#210953

Purpose

Mammalian expression of full-length human WDR12. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR12:M1-A423 (WDR12 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDR89:1-387

Plasmid

#211049

Purpose

Mammalian expression of full-length human WDR89. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR89:M1-Q387 (WDR89 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-N-RAM-d2tTA-TRE-mKate2

Plasmid

#140275

Purpose

N-RAM, the Npas4-dependent reporter

Depositor

Yingxi Lin

Article

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Has Service

AAV1

Insert

mKate2

Use

AAV

Promoter

TRE

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pminiTol2-5xUAS-hsp-sfGFP-zP2A-zN2cG-zP2A-TVA (UGNT)

Plasmid

#240273

Purpose

Helper plasmid for zebrafish rabies virus based-circuit tracing

Depositor

Xufei Du

Article

Chen et al eLife 13:RP100880

Inserts

N2cG (codon-optimized for Danio rerio)
sfGFP (codon-optimized for Danio rerio)
TVA (codon-optimized for Danio rerio)

Use

Zebrafish expression

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-F-RAM-d2tTA-TRE-mKate2

Plasmid

#140274

Purpose

F-RAM, the Fos-dependent reporter

Depositor

Yingxi Lin

Article

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Insert

mKate2

Use

AAV

Promoter

TRE

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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EF.mHES1.CMV.GFP

Plasmid

#17622

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (Hes1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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IVT-R2Tg_opt

Plasmid

#223247

Purpose

In vitro transcription of the optimized R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein_opt

Use

In vitro transcription

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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EF.hICN1.CMV.GFP

Plasmid

#17623

Purpose

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

ICN1 (NOTCH1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pGY53

Plasmid

#221709

Purpose

Empty Cas12a vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pYL-kasOp-FnCas12a2

Plasmid

#177874

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Article

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Insert

Streptomyces codon optimized FnCas12a

Use

CRISPR

Promoter

kasOp*

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

Plasmid

#176270

Purpose

Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

TadA-TadA*(8e)-dCasMINI-V4

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGY18

Plasmid

#221708

Purpose

Empty Cas9 vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA_DENV2-NS2B3_V5

Plasmid

#115906

Purpose

Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

Depositor

Alan Rothman

Article

Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

Insert

DENV2 NS2B3 protease

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcold-6xHis-HRV3Csite-HsPACT

Plasmid

#41097

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

PACT (PRKRA Human)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only