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pCS2+hSpCas9

Plasmid

#51815

Purpose

Encode hSpCas9 for in vitro transcription with Sp6

Depositor

Masato Kinoshita

Publication

Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

Insert

humanized S.pyogenes Cas9

Use

CRISPR; Medaka, zebrafish

Tags

3xFLAG

Expression

Mammalian

Promoter

SP6

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pH2B-emiRFP670

Plasmid

#136571

Purpose

Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

H2B-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-EF1a-mCherry-IRES-Flpo

Plasmid

#55634

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has service

AAV1 and AAV Retrograde

Inserts

mCherry
Flpo

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

Plasmid

#31476

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14 GFP donor EF1s I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

GFP has 14 amino acids deleted from c terminus

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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FCK-Arch-GFP

Plasmid

#22217

Purpose

Lentiviral expression of Arch-GFP for optogenetic inhibition

Depositor

Edward Boyden

Publication

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKIIa

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pCMV4-BlaM-Vpr

Plasmid

#21950

Depositor

Warner Greene

Publication

Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

Insert

BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)

Expression

Mammalian

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles
pBAD/His-miRFP670

Plasmid

#79984

Purpose

Monomeric near-infrared fluorescent protein miRFP670 in bacterial plasmid

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP670

Expression

Bacterial

Promoter

araB

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAV.CBA.eGFP.2A.P301L-Tau

Plasmid

#140425

Purpose

AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

Depositor

Bradley Hyman

Publication

Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

Insert

eGFP-2A-Tau(P301L) (MAPT Synthetic, Human)

Use

AAV

Tags

eGFP

Mutation

changed Proline 301 to Leucine

Promoter

CBA

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCDNA3-Flag MKK6

Plasmid

#13517

Depositor

Roger Davis

Publication

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6 (MAP2K6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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-3.6kb human IFN-gamma luc

Plasmid

#17599

Depositor

Howard Young

Publication

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-3.6 kb to +70 of promoter

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p3XFlag-CAPN2

Plasmid

#60942

Purpose

Expresses calpain2 in mammalian cells

Depositor

Yi Zhang

Publication

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Calpain 2 (CAPN2 Human)

Tags

3xflag

Expression

Mammalian

Promoter

CMV

Available since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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LentiGuide-Neo

Plasmid

#139449

Purpose

Lentiviral vector with gRNA scaffold and neomycin selectable marker

Depositor

Caroline Goujon

Publication

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

no sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pBiFC-bFOSDelataZipVC155

Plasmid

#22014

Depositor

Chang-Deng Hu

Publication

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bFosDelataZIPVC155

Tags

HA

Expression

Mammalian

Mutation

deletion of residues 179-193 in bFos(118-211)

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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RNT1-GFP

Plasmid

#17708

Depositor

Hal Dietz

Publication

Mendell et al Science. 2002 Oct 11. 298(5592):419-22.

Insert

rent1 (UPF1 Human)

Tags

EGFP

Expression

Mammalian

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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p3868 HA-E6-AP

Plasmid

#8648

Depositor

Peter Howley

Publication

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pmCherry-2xFYVE

Plasmid

#140050

Purpose

Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

Depositor

Harald Stenmark

Publication

Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Promoter

CMV

Available since

May 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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FLAG-Ago2

Plasmid

#21538

Depositor

Edward Chan

Publication

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

The first 7aa of this Ago2 protein are different …

Available since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pcDNA3-HA2-DDB1

Plasmid

#19909

Depositor

Yue Xiong

Publication

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Damage-specific DNA binding protein 1 (DDB1 Human)

Tags

HA

Expression

Mammalian

Mutation

please see depositor comment below

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3.1_AGIA-AirID

Plasmid

#182210

Purpose

Express AirID in mammalian cells.

Depositor

Sohei Ito, Shogo Nakano, Akira Nozawa, Tatsuya Sawasaki

Publication

Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

Insert

AGIA-AirID

Tags

AGIA-tag

Expression

Mammalian

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-GST

Plasmid

#42049

Depositor

Kumiko Ui-Tei

Publication

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

GST

Tags

His

Expression

Bacterial

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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