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SpyCatcher003-sfGFP

Plasmid

#133449

Purpose

Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003-sfGFP

Tags

His6 and TEV site

Expression

Bacterial

Promoter

T5

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pIW601-KmCRISPR

Plasmid

#98907

Purpose

K. marxianus CRISPR Plasmid for sgRNA cloning

Depositor

Ian Wheeldon

Article

Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

Inserts

Codon optimized Cas9
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Tags

SV40

Expression

Yeast

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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2C::tdTomato Reporter

Plasmid

#40281

Depositor

Samuel Pfaff

Article

Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

Insert

2C Promoter (2-cell stage promoter)

Tags

tdTomato

Expression

Mammalian

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-GFP1-10

Plasmid

#80409

Purpose

Expresses GFP1-10 in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP1-10

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiPGK DEST H2B-iRFP670

Plasmid

#90237

Purpose

Lentiviral vector to express H2B-iRFP670 under PGK promoter

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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tau/pET29b

Plasmid

#16316

Depositor

Peter Klein

Article

Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

Insert

tau (MAPT Human)

Expression

Bacterial

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only

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[#GS16-LV] Syn1-MitoTRACER - Donor

Plasmid

#233565

Purpose

Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Lentiviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMitoTimer

Plasmid

#52659

Purpose

Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

Depositor

Zhen Yan

Article

Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

Insert

DsRed1-E5

Use

Dsred

Tags

human cytochrome c oxidase subunit VIII mitochond…

Promoter

CMV

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K63

Plasmid

#17606

Purpose

Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K63 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDZ2087

Plasmid

#92414

Purpose

Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

Depositor

David Waugh

Article

Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

Insert

TEV protease

Tags

MBP (self cleaving)

Expression

Bacterial

Mutation

L56V/S135G/S219V

Promoter

tac

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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