159,589 results

1. pWN_U6-TRAC-miniGag-Cas9

   Plasmid

   #228959

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas9 fused to nanobody (anti-beta catenin)

   Plasmid

   #186420

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-nanobody fusion (anti-beta catenin)
   

   Use

   CRISPR

   Tags

   6His

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-flag-mZscan4d-polyA

   Plasmid

   #61831

   Purpose

   For mRNA synthesis or expression in mammalian cells

   Depositor

   Yi Zhang

   Article

   Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

   Insert

   Zscan4d (Zscan4d Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSirt3-RFP

   Plasmid

   #216806

   Purpose

   Human Sirt3-RFP driven by the CaMKII promoter

   Depositor

   Ghazaleh Ashrafi

   Article

   Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

   Insert

   Sirt3 (SIRT3 Human)
   

   Use

   Lentiviral

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-6His-MBP-MpAgo

   Plasmid

   #79249

   Purpose

   This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

   Depositor

   Jennifer Doudna

   Article

   Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

   Insert

   Marinitoga piezophila Argonaute
   

   Use

   CRISPR

   Tags

   6XHis-tag + MBP + PreScission Cleavage Site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX_DSB_Spectrum-V1

   Plasmid

   #192474

   Purpose

   Lentiviral DSB Spectrum V1 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, incompleteGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB441

   Plasmid

   #74827

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mKikGR-N1

   Plasmid

   #54756

   Purpose

   Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mKikGR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pDD131

   Plasmid

   #119886

   Purpose

   Optimized aTc inducible pTet promoter for Rhodococcus opacus expressing mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

   Insert

   pTet_mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223 LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid

    #215004

    Purpose

    Shuttling vector for an autophagy (LC3) reporter

    Depositor

    Wade Harper

    Insert

    MAP1LC3B (MAP1LC3B Rat)
    

    Use

    Gateway

    Tags

    HaloTag(7)-mGFP

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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