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pPB-EF1α-H2B-HaloTag-IRES-puro

Plasmid

#247460

Purpose

Piggybac based mammalian expression vector for histone H2B HaloTag, driven by the EF1a promoter.

Depositor

Kazuhiro Maeshima

Article

Hibino et al Nat Commun. 2024 Aug 21;15(1):7152. doi:

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-hATG2A

Plasmid

#114462

Purpose

Expresses human ATG2A with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

Insert

human ATG2A (ATG2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

P656R (please see depositors comment below)

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

Plasmid

#164822

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Promoter

miniCMV and pGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

Plasmid

#35509

Purpose

AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV5 and AAV9

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon TVA-mCherry

Plasmid

#131779

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

TVA-mCherry

Use

AAV; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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TurboID-NPM1c-MSCV-IRES-GFP

Plasmid

#240130

Purpose

Expresses TurboID-NPM1c fusion in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

NPM1c (NPM1 Human)

Use

Retroviral

Tags

IRES-GFP and TurboID

Expression

Mammalian

Mutation

C-terminal frameshift mutation as seen in Acute M…

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131004

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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WT Smarca4-sfGFP

Plasmid

#107056

Purpose

Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

Depositor

Jerry Crabtree, Courtney Hodges

Article

Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)

Use

Lentiviral

Tags

Super-folder GFP (sfGFP)

Expression

Mammalian

Mutation

wild-type

Promoter

CAG

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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mIFP-Alpha-Actinin-19

Plasmid

#56210

Purpose

Localization: Focal Adhesions / Actin, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-mitoX4-mVenus

Plasmid

#185808

Purpose

Mitochondrial targetted (COX8) mVenus

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

mito-mVenus

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

Plasmid

#223724

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FIP200-CTR (RB1CC1 Human)

Tags

6xHis-TEV-EGFP

Expression

Bacterial

Mutation

aa 1429-1591 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL-CAG-NR5A1-FLAG

Plasmid

#242229

Purpose

Expression of full-length human SF-1 using lentivirus

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

NR5A1 (NR5A1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

Plasmid

#35504

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO11-pcDNA3.1-

Plasmid

#52501

Purpose

expresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO11 (FBXO11 Human)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCas-Cas12m

Plasmid

#192274

Purpose

Encodes MmCas12m under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12

Use

CRISPR

Expression

Bacterial

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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Luciferase

Plasmid

#213979

Purpose

Expresses Doxy Inducible Luciferase

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

Luciferase

Use

Lentiviral

Tags

6xHis affinity tag

Expression

Bacterial and Mammalian

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Fon EYFP

Plasmid

#55650

Purpose

Cre-on/Flp-on EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Insert

eYFP with introns

Use

AAV; Cre on/flp on eyfp

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pBMN BFP-Parkin

Plasmid

#186221

Purpose

For expression of Parkin tagged with BFP

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

Parkin (PRKN Human)

Use

Retroviral

Tags

BFP

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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HAP40 1-371

Plasmid

#124060

Purpose

Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HAP40 (F8A1 Human)

Use

Baculovirus expression

Tags

6x His and TEV cleavage site

Promoter

polyhedrin

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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