We narrowed to 3,617 results for: mak

1. pCAGG-GST-TEV-NAP1 (WT)

   Plasmid

   #198034

   Purpose

   Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

   Plasmid

   #198072

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   TBK1ΔCTD (res 1-666)

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGST2-GST-TEV-OPTN S177D S473D

   Plasmid

   #187827

   Purpose

   Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   OPTN (OPTN Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Bacterial

   Mutation

   S177D S473D

   Promoter

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-mitoX4-mVenus

   Plasmid

   #185808

   Purpose

   Mitochondrial targetted (COX8) mVenus

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   mito-mVenus
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-mitoX4-FKBP-HA

   Plasmid

   #185658

   Purpose

   Mitochondrial targetted (Cox8) FKBP protein with C-terminal HA epitope

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   mito-FKBP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBacMam2-DiEx-LIC-N entry vector

   Plasmid

   #111751

   Purpose

   Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-PIG-N3FLAG-TAF12

   Plasmid

   #105593

   Purpose

   retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
   

   Use

   Retroviral

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   silent mutations to make it resistant to the targ…

   Promoter

   MSCV-LTR

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. RGS-6xHis-LIN-29-pcDNA3.1-

   Plasmid

   #52515

   Purpose

   expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Lin-29 b (lin-29 Nematode)
   

   Use

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Mutation

   wt (changed coding sequence in 5' (5th codon…

   Promoter

   CMV

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mIFP-Cx26-7

   Plasmid

   #56216

   Purpose

   Localization: Gap Junctions, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   Cx26 (Gjb2 Rat)
   

   Use

   Tags

   mIFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCS2+MLS-HyPer7

    Plasmid

    #136470

    Purpose

    Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Tandem mitochondrial targeting signal of cytochro…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCE-mp53DD

    Plasmid

    #41856

    Purpose

    Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CAG

    Available since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+HyPer7-NES

    Plasmid

    #136467

    Purpose

    Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Nuclear export signal from the HIV Rev protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCE-hUL

    Plasmid

    #41855

    Purpose

    Non-integrating (episomal) expression of human L-MYC and LIN28

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    L-MYC, LIN28 (MYCL, LIN28A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRRL_SRSF2_P95H_mCherry

    Plasmid

    #84023

    Purpose

    expression of SRSF2 mutant in mammalian cells

    Depositor

    Robert Bradley

    Article

    Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

    Insert

    SRSF2 (SRSF2 Human)
    

    Use

    Lentiviral

    Tags

    2TA-mCherry and Flag

    Expression

    Mammalian

    Mutation

    P95H

    Promoter

    PGK

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-FuG-B2

    Plasmid

    #67513

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.

    Insert

    Fusion Glycoprotein type B2
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-hSyn-ASAP4e-Kv-WPRE

    Plasmid

    #201031

    Purpose

    Expresses somatically enriched ASAP4e in neurons, can be used to package AAV

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4e-Kv
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2+HyPer7-NLS

    Plasmid

    #136468

    Purpose

    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Triple nuclear localization signal of SV40 (simia…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. eGFP-proα2(I)

    Plasmid

    #119826

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-5 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR_SFFv_Her2-BFP_RHL027

    Plasmid

    #164823

    Purpose

    Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    Extracellular and transmembrane region of ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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