We narrowed to 6,981 results for: Mag

Showing: 4841 - 4880 of 6981 results

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pME-Myc-BioID2-MCS1 (JDW 1095)

Plasmid

#224510

Purpose

Gateway compatible middle entry clone containing a Myc-tagged BIOID2 construct

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BioID2

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-H2A_mCherry_SV40pA (JDW 967)

Plasmid

#224528

Purpose

A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2A-mCherry-SV40pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-V5-mClover3-pA (JDW 1318)

Plasmid

#224530

Purpose

A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mClover3-pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-mTagBFP2-pA (JDW 1361)

Plasmid

#224535

Purpose

A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-FLAG-NLS-mTagBFP2-pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)

Plasmid

#224537

Purpose

A Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-(n2)StayGold(c4)-pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME H2B mCerulean (JDW 1150)

Plasmid

#224543

Purpose

A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mCerulean

Use

Gateway cloning

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-miRFP670

Plasmid

#220600

Purpose

Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

miRFP670

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

Plasmid

#220597

Purpose

Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mVenus-Q69M (ME)

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mKate2

Plasmid

#220599

Purpose

Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-ArgiNLS-EGFP

Plasmid

#220601

Purpose

Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

EGFP

Use

AAV and Cre/Lox

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-FOXA3

Plasmid

#219395

Purpose

Express FOXA3 in mammalian cells

Depositor

Yoichi Furukawa

Article

Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

Insert

FOXA3 (FOXA3 Human)

Tags

N terminal Myc tag

Expression

Mammalian

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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HCA3-DuET

Plasmid

#213309

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

HCA3 (HCAR3 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXC4-WT

Plasmid

#215586

Purpose

For overexpression of Gal4-DBD HOXC4 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXC4 (HOXC4 Human)

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-PDX1_AroPERFECT

Plasmid

#215644

Purpose

For overexpression of Gal4-DBD PDX1 AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

PDX1 AroPERFECT (PDX1 Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-MYOD1_AroLITE

Plasmid

#215618

Purpose

For integration of MYOD1 AroLITE T2A mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

MYOD1 AroLITE (MYOD1 Human)

Tags

T2A mEGFP

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-NFAT5-AroLITE_A

Plasmid

#215598

Purpose

For overexpression of Gal4-DBD NFAT5 AroLITE A

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NFAT5 AroLITE A (NFAT5 Human)

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-MYOD1_AroLITE-C

Plasmid

#215619

Purpose

For integration of MYOD1 AroLITE C T2A mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

MYOD1 AroLITE C (MYOD1 Human)

Tags

T2A mEGFP

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-PDX1_AroLITE

Plasmid

#215643

Purpose

For overexpression of Gal4-DBD PDX1 AroLITE

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

PDX1 AroLITE (PDX1 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-PDX1_WT

Plasmid

#215642

Purpose

For overexpression of Gal4-DBD PDX1 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

PDX1 (PDX1 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-NANOG-WT

Plasmid

#215599

Purpose

For overexpression of Gal4-DBD NANOG WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NANOG (NANOG Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-FOXA3_WT

Plasmid

#215639

Purpose

For overexpression of Gal4-DBD FOXA3 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

FOXA3 (FOXA3 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv_V50A

Plasmid

#183734

Purpose

Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Mutation

V50A mutation in MFalpha signal

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Amber-free HIV-1 deltaRT

Plasmid

#213007

Purpose

An replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.

Depositor

Maolin Lu

Article

Ao et al Cell Chem Biol. 2024 Jan 8:S2451-9456(23

Inserts

HIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)
Env BG505

Use

Unspecified

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Qt-FLAG-TGA-rt9us-antiGFP

Plasmid

#206954

Purpose

Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

QtEnc, antiGFP-intrabody

Expression

Mammalian

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Qt-FLAG-TAA-rt20s-antiGFP

Plasmid

#206955

Purpose

Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

QtEnc, antiGFP-intrabody

Expression

Mammalian

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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1M-Qt-FLAG-TGA-rt20s-antiGFP

Plasmid

#206953

Purpose

Mammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc, antiGFP-intrabody

Expression

Mammalian

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-AcGFP-CI

Plasmid

#196849

Purpose

Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

AcGFP

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-NFYB

Plasmid

#170697

Purpose

doxycycline-inducible overexpression of NFYB

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

NYFB (NFYB Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

Plasmid

#196877

Purpose

Neuron-specific expression of mOrange2 reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-td-mCherry-CI

Plasmid

#196851

Purpose

Expression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

td-mCherry

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2

Plasmid

#185346

Purpose

Membrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Cue1p/Ube2G2 fusion (internal MYC tag)

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG

Plasmid

#185347

Purpose

Membrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tag

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Cue1p/Ube2G2 fusion (internal MYC tag)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_139

Plasmid

#180565

Purpose

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry-Cenexin-S796A

Plasmid

#186434

Purpose

Expresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

Insert

Cenexin (ODF2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Changed Serine 796 to Alanine

Promoter

CMV IE94

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_113

Plasmid

#180540

Purpose

Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Signal peptide region of CsgA including SecB-N22 sequenced

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251_ Ptrc.x.tetO2::D-HicDH

Plasmid

#185456

Purpose

CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

Depositor

Wolfgang Kroutil

Article

Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

Insert

D-HicDH from Lactobacillus paracasei DSM 20008

Expression

Bacterial

Promoter

Ptrc.x.tetO2

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_095

Plasmid

#180524

Purpose

dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_324

Plasmid

#180566

Purpose

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
iDianiSnFR

Plasmid

#177741

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iDianiSnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG660 lyn11-pFAST

Plasmid

#172863

Purpose

expresses lyn11-pFAST in mammalian cells (inner membrane labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

Lyn11-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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