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We narrowed to 10,458 results for: T7

Showing: 2421 - 2440 of 10458 results

  1. pT7CFE1_polio_3ABC_C147R

    Plasmid
    #212985
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    cysteine 147 in 3C changed to arginine
    Promoter
    T7
    Available since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212985-plasmid-map-sequence-id-422575

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  2. pT7CFE1_polio_VP12A_C109R

    Plasmid
    #212987
    Purpose
    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney VP12A C109R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Cysteine 109 from 2A mutated to arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212987-plasmid-map-sequence-id-420359

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  3. pET28-RPL3-Twstrep

    Plasmid
    #208623
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) (RPL3 Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208623-plasmid-map-sequence-id-418316

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  4. TDRD3:JMC006-H10:C42283

    Plasmid
    #183981
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD3 in Complex with anti-TDRD3 FAB (TDRD3 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183981-plasmid-map-sequence-id-361433

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  6. pET28a-RupA_T1-NHis

    Plasmid
    #109259
    Purpose
    NRPS T domain with N-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_T1
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    contains aa 917..1025 of WP_015523502.1
    Promoter
    T7
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109259-plasmid-map-sequence-id-211504

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  7. pET28a-RupA_R-NHis

    Plasmid
    #109257
    Purpose
    NRPS R domain with N-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_R
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    contains aa 2038..2443 of WP_015523502.1
    Promoter
    T7
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109257-plasmid-map-sequence-id-211503

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  8. WNK2 (WNK2A-c003)

    Plasmid
    #110250
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    WNK2 (WNK2 Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 - lacO
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110250-plasmid-map-sequence-id-212284

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  9. 5JJW (USP15_SART3)

    Plasmid
    #101880
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15_SART3 (USP15, SART3 Human)
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101880-plasmid-map-sequence-id-194708

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  11. His 3C MKK3 42-347

    Plasmid
    #87879
    Purpose
    E. coli expression of MKK3 aa 42-347
    Depositor
    Peter Mace
    Insert
    MKK3 (MAP2K3 Human)
    Use
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 42-347
    Promoter
    T7
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87879-plasmid-map-sequence-id-174922

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  12. pHL 2146

    Plasmid
    #69789
    Purpose
    KanR with FRT sites + pT7::SgrS::T1 terminator + ColE
    Depositor
    Han Lim
    Article
    Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw
    Insert
    SgrS
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. BRD1A

    Plasmid
    #74655
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD1A (BRD1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. pJK559

    Plasmid
    #71603
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71603-plasmid-map-sequence-id-131677

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  15. pJK562

    Plasmid
    #71604
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71604-plasmid-map-sequence-id-131680

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  16. pJK604

    Plasmid
    #71610
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71610-plasmid-map-sequence-id-131686

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  17. pJK664

    Plasmid
    #71613
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tyrosine-154 …
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71613-plasmid-map-sequence-id-131691

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  18. pJK665

    Plasmid
    #71614
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp97>Tyr mutant (AaPurE1fyw-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71614-plasmid-map-sequence-id-131692

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  19. pJK666

    Plasmid
    #71615
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-97…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71615-plasmid-map-sequence-id-131694

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  20. pJK563

    Plasmid
    #71689
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71689-plasmid-map-sequence-id-131681

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Showing: 2421 - 2440 of 10458 results