We narrowed to 12,157 results for: pcdna

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pcDNA3-C16orf59-mVen

Plasmid

#211425

Purpose

mammalian expression of human C16orf59 C-terminally fused to mVenus

Depositor

Helmut Pospiech

Insert

C16orf59 (C16orf59 Human)

Tags

mVenus

Expression

Mammalian

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApaLIE225A, K227A-HA

Plasmid

#211460

Purpose

Express mitochondrial localized ApaLI with E225A and K227A mutations

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-E225A-K227A

Tags

HA

Expression

Mammalian

Mutation

E225A, K227A

Promoter

CMV

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hFCER1G-VenusFlag

Plasmid

#211899

Purpose

Expresses human FCER1G in mammalian cells

Depositor

Haian Fu

Insert

FCER1G (FCER1G Human)

Tags

VenusFlag

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Ubc13

Plasmid

#212806

Purpose

Constitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc13

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Mms2

Plasmid

#212807

Purpose

Constitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Mms2

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA K230A

Plasmid

#210811

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PuRRRE

Plasmid

#208327

Purpose

C-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PuRRRE

Tags

PuRRRE

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TREER

Plasmid

#208325

Purpose

C-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TREER

Tags

TREER

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TinyERRE

Plasmid

#208324

Purpose

C-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TinyERRE

Tags

TinyERRE

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-N

Plasmid

#210270

Purpose

N-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Type

Empty backbone

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 BBS pcDNA3

Plasmid

#206106

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit with a single Bungarotoxin Binding Site motif inserted after aa549HA

Depositor

Annette Dolphin

Article

Meyer et al Sci Rep. 2021 May 13;11(1):10256. doi: 1

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PunyEEER

Plasmid

#208326

Purpose

C-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PunyEEER

Tags

PunyEEER

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-G671R-N865K

Plasmid

#203699

Purpose

expresses ATP10B with the pathogenic mutations G671R and N865K

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Mutation

G671R, N865K

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 W391A pcDNA3

Plasmid

#206085

Purpose

expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hARFRP1(T31N)-HA

Plasmid

#206401

Purpose

Mammalian expression of hARFRP1(T31N)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

Insert

ARFRP1 (ARFRP1 Human)

Tags

HA

Expression

Mammalian

Mutation

T31N

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hARFRP1(Q79L)-HA

Plasmid

#206400

Purpose

Mammalian expression of hARFRP1(Q79L)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

Insert

ARFRP1 (ARFRP1 Human)

Tags

HA

Expression

Mammalian

Mutation

Q79L

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrasnatQ61R

Plasmid

#206843

Purpose

To express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Glutamine 61 to Arginine

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2-Venus

Plasmid

#203712

Purpose

Constitutive mammaliam expression of human NKX6-2 cDNA fused with the Venus fluorescent protein

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 fused with Venus (NKX6-2 Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2(1-40)-Venus

Plasmid

#203713

Purpose

SPAX8-related truncated mutation (K41*) containing the amino acids 1 to 40 of human NKX6-2, fused with the Venus fluorescent protein.

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (1-40) fused with Venus (NKX6-2 Human)

Tags

Venus

Expression

Mammalian

Mutation

deletion of amino acids 41-277

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Rat MMP-9 pcDNA3.1

Plasmid

#196951

Purpose

Plasmid expressing an antigen targeting the MMP9 metalloproteinase

Depositor

James Trimmer

Insert

mmp-9 (Mmp9 Rat)

Expression

Mammalian

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA W391A pcDNA3

Plasmid

#206071

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 HA D491A pcDNA3

Plasmid

#206070

Purpose

expression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin binding

Depositor

Annette Dolphin

Article

Gumerov et al Proc Natl Acad Sci U S A. 2022 Mar 8;119

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

D491A

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 - circMAN2A1

Plasmid

#198837

Purpose

This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

Depositor

Stefan Stamm

Article

Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

Insert

circular mannosidase alpha class 2A member 1

Expression

Mammalian

Mutation

circular RNA composed by 2 exons

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-INSRR-V5/HIS

Plasmid

#201981

Purpose

expression of human INSRR receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

INSRR receptor tyrosine kinase (INSRR Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB

Plasmid

#193808

Purpose

CMV overexpression of ChemoB in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB

Tags

10xHis

Expression

Mammalian

Mutation

EBFP2: N39Y, V206K, T225R; HT7: E143R, E147R, L27…

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoY

Plasmid

#193810

Purpose

CMV overexpression of ChemoY in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoY

Tags

10xHis

Expression

Mammalian

Mutation

Venus:A206K, T225R; HT7: E143R, E147R, L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoR

Plasmid

#193811

Purpose

CMV overexpression of ChemoR in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoR

Tags

10xHis

Expression

Mammalian

Mutation

mScarlet: D201K

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-nsp7

Plasmid

#201026

Purpose

Expresses SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Expression

Mammalian

Promoter

T7 promoter

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-nsp7

Plasmid

#201025

Purpose

Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Expression

Mammalian

Promoter

CMV-tet-ON

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1 A397D

Plasmid

#185342

Purpose

Assessing G2BR function in AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

Flag, 6xHis

Expression

Mammalian

Mutation

A397D

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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