We narrowed to 22,746 results for: Sele

1. pGEX-4T1-USP11

   Plasmid

   #78849

   Purpose

   To purify GST-tagged recombinant USP11 protein

   Depositor

   Jaewhan Song

   Article

   Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

   Insert

   USP11 (USP11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

2. OPRD1-DuET

   Plasmid

   #213358

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   OPRD1 (OPRD1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Teto Ngn2

   Plasmid

   #62223

   Purpose

   Inducible expression of Ngn2

   Depositor

   Kristin Baldwin

   Article

   Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi:

   Insert

   Neurog2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJT130_GalL_nCDA1-NGBE3

   Plasmid

   #145094

   Purpose

   Expressing base editor nCDA1-NGBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-NGBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MEF2D-2A-neo

   Plasmid

   #190622

   Purpose

   Lentiviral expression plasmid of human MEF2D (long isoform), neomycin selection

   Depositor

   Junwei Shi

   Article

   Pingul et al iScience. 2022 Sep 15;25(10):105139. doi

   Insert

   MEF2D (MEF2D Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SSTR2-DuET

   Plasmid

   #213377

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   SSTR2 (SSTR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCWXPG-UBI-MafA

   Plasmid

   #114302

   Purpose

   Constitutive expression of the MafA trancription factor

   Depositor

   Patrick Salmon

   Article

   Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

   Insert

   MafA (MAFA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCEP4-myc-HLA-A1

   Plasmid

   #135503

   Purpose

   Mammalian expression of myc-tagged HLA-A*01:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*01:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 4LNDDE488Q-pCDNA3.1(+)

   Plasmid

   #172228

   Purpose

   Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

   Depositor

   Joshua Rosenthal

   Article

   Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain E488Q
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   changed the Glutamic acid 488 in the Deaminase Do…

   Promoter

   CMV

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid

    #102885

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDH-SIRT2-Flag

    Plasmid

    #102624

    Purpose

    Expresses full length SIRT2 isoform 1 in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3 Flag Jnk2a2(apf)

    Plasmid

    #13761

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2a2(apf) (MAPK9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced dual phosporylation motif THr(183)-Pro-T…

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Syn-AlstR-IRES2-EGFP

    Plasmid

    #14895

    Depositor

    Edward Callaway

    Article

    Tan et al Neuron. 2006 Jul 20. 51(2):157-70.

    Insert

    AlstR (AstA-R1 Fly)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MTNR1A-DuET

    Plasmid

    #213348

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MTNR1A (MTNR1A Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLP618_αGCN4-KRAB-CO

    Plasmid

    #211782

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-mCherry
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect mCherr…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSFDuet-1 IntS8 AA 1-308

    Plasmid

    #196905

    Purpose

    Expresses His-tagged Drosophila IntS8 amino acids 1-308

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 8 (IntS8 Fly)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. JN302: pMVP/SB/Puro-DEST

    Plasmid

    #121865

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-FLAG-APEX2-CALCOCO2

    Plasmid

    #175759

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-CALCOCO2/NDP52

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    NDP52/CALCOCO2 (CALCOCO2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-FLAG-APEX2-LGALS1

    Plasmid

    #175755

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS1

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS1 (LGALS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3 Flag MKK3b(Glu)

    Plasmid

    #50449

    Purpose

    activates p38 in the signaling pathway

    Depositor

    Roger Davis

    Article

    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

    Insert

    MKK3b (MAP2K3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Serine 218 to Glutamic Acid, changed Thre…

    Promoter

    CMV

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only