We narrowed to 17,100 results for: emb

1. pCDH-EF1a-HygroR-T2A-TetOn3G

   Plasmid

   #179887

   Purpose

   Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   TetOn3G
   

   Use

   Lentiviral

   Tags

   Hygromycin resistance

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV103

   Plasmid

   #63193

   Purpose

   pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Worm and Yeast

   Available Since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1_Cas9-hGem_gRNA2

   Plasmid

   #217659

   Purpose

   expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA2 targeting the human AAVS1 safe harbour locus
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBTK402

   Plasmid

   #110598

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. TrpLE-M-nMSP1E3D1

   Plasmid

   #179949

   Purpose

   Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

   Depositor

   Kerney Glover

   Article

   Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

   Insert

   TrpLE-M-nMSP1E3D1
   

   Tags

   TrpLE

   Expression

   Bacterial

   Mutation

   Native Trp residues have been mutated to Phe resi…

   Promoter

   T7

   Available Since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-UBC-TagRFP-T-ps

   Plasmid

   #178656

   Purpose

   Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

   Insert

   TagRFP-T with palmitoylation signal at N-terminus
   

   Use

   Lentiviral

   Tags

   Palmitoylation signal

   Promoter

   Ubc

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWaldo-GFPe_PepTSo

   Plasmid

   #58334

   Purpose

   Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

   Depositor

   So Iwata, Simon Newstead

   Article

   Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

   Insert

   PepTso
   

   Tags

   GFP-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Fyn-Fyn Biosensor

   Plasmid

   #124655

   Purpose

   FRET biosensor. Fyn-tagged biosensor targeting to membrane

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

   Insert

   Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBTK501

   Plasmid

   #110602

   Purpose

   BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBTK229

    Plasmid

    #110591

    Purpose

    BTK Type 3 coding sequence for golden gate assembly and homologous recombination

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Kanamycin resistance
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBTK570

    Plasmid

    #110615

    Purpose

    BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    E2-Crimson
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA3-AktAR-Kras

    Plasmid

    #123357

    Purpose

    FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

    Depositor

    Jin Zhang

    Article

    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

    Insert

    AktAR-Kras
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBTK520

    Plasmid

    #110604

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Tmem214 pmRFP-N2 (1348)

    Plasmid

    #62047

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

15. VAMP2-12aa-mCit

    Plasmid

    #53237

    Purpose

    Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    12aa-linker and mCitrine

    Expression

    Mammalian

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pBTK510

    Plasmid

    #110611

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pMV_AA090

    Plasmid

    #216099

    Purpose

    Knockout, EF1a-driven Cas12a (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas12a [EnAs]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBTK564

    Plasmid

    #110613

    Purpose

    BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Nanoluc
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pTHMT_hnRNPF_FL

    Plasmid

    #139114

    Purpose

    express FL hnRNPF with N-terminal maltose binding protein tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPF (HNRNPF Human)
    

    Expression

    Bacterial

    Mutation

    None

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tmem214 pEGFP-N2 (1072)

    Plasmid

    #62026

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only