We narrowed to 6,824 results for: pcDNA3.1+

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pcDNA3.1/Hygro-Mfn2-ActA

Plasmid

#191981

Purpose

Expression of Mfn2 tagged with mitochondrion localization signal (ActA)

Depositor

Luca Scorrano

Insert

Mfn2-ActA

Tags

ActA

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-mEGFP-pp4640

Plasmid

#191846

Purpose

Expresses Salmon Alphavirus polyprotein with mEGFP N-terminal fusion

Depositor

Bertrand Collet

Article

Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

Insert

mEGFP-pp4640

Tags

mEGFP

Expression

Mammalian

Mutation

mEGFP fusion in Nterminal, some silent point muta…

Promoter

CMV

Available Since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RIα(D/D+linker)-EGFP

Plasmid

#181842

Purpose

EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Contains only residues 12-112 of PKA RIα.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

Plasmid

#177771

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K370A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Asp115Ala

Plasmid

#180760

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Asp115Ala

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Asp115Ala, Leu…

Promoter

CMV

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRBP-L1+D1+L2-myc/His

Plasmid

#180599

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/TO-myc-BirA*-cCE

Plasmid

#112713

Purpose

For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Tags

BirA* (BirA R118G), HIV Rev nuclear localization …

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBIV

Plasmid

#176055

Purpose

Expresses Myc Mutant (delta Myc BoxIV) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 304-324

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBIIb

Plasmid

#176054

Purpose

Expresses Myc Mutant (delta Myc BoxIIb) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 259-270

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBIIa

Plasmid

#176053

Purpose

Expresses Myc Mutant (delta Myc BoxIIa) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 188-199

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBII

Plasmid

#176052

Purpose

Expresses Myc Mutant (delta Myc BoxII) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 128-143

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBI

Plasmid

#176051

Purpose

Expresses Myc Mutant (delta Myc BoxI) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 44-63

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMB0

Plasmid

#176050

Purpose

Expresses Myc Mutant (delta Myc Box0) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 16-33

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CDK4

Plasmid

#172615

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK4 (CDK4 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_DED/AAA USP7

Plasmid

#131253

Purpose

Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Contains point mutations that convert USP7 aspart…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP EMI1

Plasmid

#158947

Purpose

Express human EMI1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

EMI1 (FBXO5 Human)

Tags

2X FLAG 2X STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1.miR17

Plasmid

#128748

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-17 site inserted in mCherry 3'UTR.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with miR-17 site replacing miR-7 site (OIP5-AS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-V5H6-mPCSK3 D153A

Plasmid

#122681

Purpose

Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Expression

Mammalian

Mutation

changed Aspartic Acid 153 to Alanine

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-Lock-VWF

Plasmid

#124824

Purpose

Expresses multimeric human VWF protein without A1 domain and Lock A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and Lock A2 domain (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Char-BFP2-TSERex

Plasmid

#124792

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Char-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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