We narrowed to 9,183 results for: try;

1. pET22b_PstS_3

   Plasmid

   #78200

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,I76G,A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A17C,I76G,A197C (please see depositor comment bel…

   Promoter

   T7

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b_PstS_2

   Plasmid

   #78199

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A17C; A197C (please see depositor comment below)

   Promoter

   T7

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-10xSunTag-P2A-tagBFP

   Plasmid

   #174141

   Purpose

   Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, 10xSunTag, tagBFP
   

   Use

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMVHA E2F1

   Plasmid

   #24225

   Purpose

   Mammalian expression of human E2F1 with HA tag

   Depositor

   Kristian Helin

   Article

   Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

   Insert

   E2F1 (E2F1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. prDNA spacer-LacOx50-20xMS2-Hyg

   Plasmid

   #210219

   Purpose

   Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   50xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20942

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   EF1alpha

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. MT1E

   Plasmid

   #105702

   Purpose

   Bacterial expression plasmids for production of recombinant metallothionein 1e

   Depositor

   Artur Krężel

   Article

   Peris-Diaz et al J Proteome Res. 2021 Jan 1;20(1):776-785

   Insert

   Metallothionein 1e (MT1E Human)
   

   Use

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR223-BALF4

   Plasmid

   #195835

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BALF4

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BALF4 (BALF4 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. 612 p3E-2A-mCherryCAAXpA

   Plasmid

   #195971

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-mCherry-CAAX (membrane)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ220-Ultra

    Plasmid

    #176889

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

    Insert

    AsCas12a
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-Nup35-mut

    Plasmid

    #87341

    Purpose

    To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP35 (NUP35 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSNRW-Z5-Nluc

    Plasmid

    #174487

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z5-Nluc
    

    Use

    Tags

    6x Histidine tag (N terminal on insert)

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_HUWE1_isoform1

    Plasmid

    #190464

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3_LuxSit_i_mTagBFP2

    Plasmid

    #194300

    Purpose

    Mammalian expression of the de novo luciferase, LuxSit-i

    Depositor

    David Baker, Andy Yeh

    Article

    Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

    Insert

    LuxSit-i
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJB02

    Plasmid

    #111959

    Purpose

    Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Inserts

    mEGFP
    eDHFR
    

    Use

    Tags

    DHFR

    Expression

    Bacterial

    Mutation

    eDHFR;residues 2-159 and enhanced GFP with monome…

    Promoter

    T7

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET51b-His-TEV-SNAP-tag

    Plasmid

    #167269

    Purpose

    Overexpression of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLeAPS-GFP-blast

    Plasmid

    #182230

    Purpose

    Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    GFP-P2A-Blasticidin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC-NRP1

    Plasmid

    #158384

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    NRP1 (NRP1 Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. 766 p3E-2A-nlsmCherrypA

    Plasmid

    #195972

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Kristen Kwan, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-nlsmCherry (nuclear)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET51b-His-TEV-CLIP-tag

    Plasmid

    #167274

    Purpose

    Overexpression of CLIP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    CLIP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge