Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 8,780 results for: construct

Clear keywords
Showing: 4921 - 4960 of 8780 results

  2. GARS_pNIC-Bio3

    Plasmid
    #153049
    Purpose
    Expression construct for producing Avi tagged Aspartyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    GARS (GARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153049-plasmid-map-sequence-id-304463

    Enlarge

  6. pEGB3_alpha2 35:PhyB:VP16 (GB2745)

    Plasmid
    #160630
    Purpose
    TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch
    Depositor
    Diego Orzaez
    Insert
    PhyBVP16
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available Since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160630-plasmid-map-sequence-id-321969

    Enlarge

  12. Tet-O-FUW-OVOL2-PURO

    Plasmid
    #251735
    Purpose
    Overexpression construct expressing OVOL2-PURO and ORF-BC
    Depositor
    Andrea Califano
    Insert
    OVOL2 (OVOL2 Human)
    Use
    Lentiviral
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251735-plasmid-map-sequence-id-501480

    Enlarge

  13. pLFYa

    Plasmid
    #243803
    Purpose
    Dropout vector for genomic integration into yeast strain LFYa. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains the attP site for the serine recombinase BxB1.
    Depositor
    Sai Reddy
    Article
    Lester et al bioRxiv
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    243803-plasmid-map-sequence-id-487915

    Enlarge

  14. pLFYalpha

    Plasmid
    #243804
    Purpose
    Dropout vector for genomic integration into yeast strain LFYalpha. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains attB site for the serine recombinase BxB1.
    Depositor
    Sai Reddy
    Article
    Lester et al bioRxiv
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    243804-plasmid-map-sequence-id-487916

    Enlarge

  15. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195623
    Purpose
    Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195623-plasmid-map-sequence-id-385117

    Enlarge

  16. (942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195624
    Purpose
    pAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Use
    AAV
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195624-plasmid-map-sequence-id-385116

    Enlarge

  17. pMX-Flag-GFP_EDIL3

    Plasmid
    #227957
    Purpose
    Control plasmid. Important note: Construct with N-terminally tagged GFP which might mask the signal sequence for proper localization of the protein
    Depositor
    Alessandro Ori
    Insert
    EDIL3 (EDIL3 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Available Since
    Feb. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    227957-plasmid-map-sequence-id-461332

    Enlarge

  18. Rab11A[8-175]-His-C

    Plasmid
    #228040
    Purpose
    Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling.
    Depositor
    Wade Harper
    Insert
    Rab11A[8-175]-His-C (RAB11A Human)
    Expression
    Bacterial
    Available Since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    228040-plasmid-map-sequence-id-450386

    Enlarge

  19. pMta2-EosHalo-TAFiP

    Plasmid
    #140959
    Purpose
    Targeting construct to make Mta2-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    metastasis-associated gene family, member 2 (Mta2 Mouse, Synthetic)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    140959-plasmid-map-sequence-id-338415

    Enlarge

  26. pCASCADE-gltA1-gltA2-udhA-GapAP1

    Plasmid
    #87151
    Purpose
    gltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-GapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87151-plasmid-map-sequence-id-174940

    Enlarge

  28. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

    Enlarge

  29. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

    Enlarge

  30. pSLIK DNM1L-V5 neo

    Plasmid
    #220363
    Purpose
    Lentiviral expression vector for an inducible V5-tagged DNM1L construct
    Depositor
    Kevin Janes
    Insert
    DNM1L (DNM1L Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220363-plasmid-map-sequence-id-436888

    Enlarge

  37. LARS_pNIC-Bio3

    Plasmid
    #153052
    Purpose
    Expression construct for producing Avi tagged Leucyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    LARS (LARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Not full length protein
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153052-plasmid-map-sequence-id-304468

    Enlarge

  40. Tet-O-FUW-OVOL2-3xFLAG-PURO

    Plasmid
    #251740
    Purpose
    Overexpression construct expressing OVOL2-3xFLAG-PURO and ORF-BC
    Depositor
    Andrea Califano
    Insert
    OVOL2-3xFLAG (OVOL2 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251740-plasmid-map-sequence-id-501484

    Enlarge

Showing: 4921 - 4960 of 8780 results