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pET28a-DDX43

Plasmid

#134574

Purpose

Express human DDX43 protein (WT, full-length) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43 (DDX43 Human)

Tags

His

Expression

Bacterial

Mutation

full-length WT

Promoter

T7

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-NMHC-IIA-S1916A

Plasmid

#101040

Purpose

Expresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.

Depositor

Tom Egelhoff

Article

Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311

Insert

Non-muscle myosin IIA heavy chain (MYH9 Human)

Tags

GFP

Expression

Mammalian

Mutation

S1916A, S1917A

Promoter

CMV

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Mito-7

Plasmid

#57795

Purpose

Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Mito (COX8A Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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Mouse aB crystallin 1.5kb promoter/AcGFP

Plasmid

#98104

Purpose

Mouse aB crystallin 1.5kb promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha B-crystallin promoter 1.5 kb fragment, Mus musculus (Cryab Mouse)

Use

Gfp expressing

Tags

GFP

Promoter

Alpha B-crystallin 1.5 kb promoter fragment (-150…

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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INS-GFP-CD19-EPT

Plasmid

#210469

Purpose

Donor plasmid for knock-in GFP-CD19 into the human INS locus

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2483

Plasmid

#142104

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CBX2 (CBX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-K292A

Plasmid

#134575

Purpose

Express human DDX43 mutant (K292A, ATPase dead) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-K292A (DDX43 Human)

Tags

His

Expression

Bacterial

Mutation

full-length (K292A)

Promoter

T7

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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His-13bCTS-GFP

Plasmid

#247243

Purpose

A bacterial expression plasmid encoding ciliary targeting sequence of mammalian Arl13b (amino acids 347-363) with N-terminal His and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

deleted amino acid 1-346,364-428

Promoter

T7 Promoter

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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CD8a-AA354-363-GFP

Plasmid

#221599

Purpose

Expresses CD8a fused with a segment of the Arl13b protein (amino acids 354-363) and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 354-363 are inserted

Promoter

CMV promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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CD8a-AA354-361-GFP

Plasmid

#247762

Purpose

Expresses CD8a fused with a segment of the Arl13b protein (amino acids 354-361) and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 354-361 are inserted

Promoter

CMV promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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CD8a-AA358-361-GFP

Plasmid

#247767

Purpose

Expresses CD8a fused with a segment of the Arl13b protein (amino acids 358-361) and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13b (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 354-361 are inserted

Promoter

CMV promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4b)

Plasmid

#241044

Purpose

Expresses SARS-CoV-2 spike (B.1-4b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,K529L,D614G,R683L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4c)

Plasmid

#241045

Purpose

Expresses SARS-CoV-2 spike (B.1-4c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

N439R,T478K,E484V,D614G,K529L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4a)

Plasmid

#241042

Purpose

Expresses SARS-CoV-2 spike (B.1-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,F490R,D614G,R683N

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4d)

Plasmid

#241043

Purpose

Expresses SARS-CoV-2 spike (B.1-4d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2c)

Plasmid

#241040

Purpose

Expresses SARS-CoV-2 spike (B.1-2c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2d)

Plasmid

#241041

Purpose

Expresses SARS-CoV-2 spike (B.1-2d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-TSC22D1-Brd 140

Plasmid

#219104

Purpose

Transcription factor TSC22D1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

TSC22D1 (TSC22D1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-VSIG2-COMP5AP-AviTag-9xHis

Plasmid

#157223

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VSIG2 (VSIG2 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 [315-445]

Plasmid

#186474

Purpose

Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [315-445]

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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