We narrowed to 9,006 results for: Pol;

1. eDrEGFR

   Plasmid

   #184000

   Purpose

   Optically controlled eDrEGFR expression in mammalian cells

   Depositor

   Vladislav Verkhusha, Dmitry Gerashchenko

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrEGFR (EGFR Human)
   

   Use

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_SLC39A8

   Plasmid

   #132177

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

   Insert

   SLC39A8 (SLC39A8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV-CaMKII-pAceR-Kv2.1PR

   Plasmid

   #195531

   Purpose

   Red fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAceR-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   VARNAM 78E, 81D, 92N; SI linker

   Promoter

   CaMKII

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. eDrcKIT

   Plasmid

   #184001

   Purpose

   Optically controlled eDrcKIT expression in mammalian cells

   Depositor

   Vladislav Verkhusha, Dmitry Gerashchenko

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrcKIT (KIT Human)
   

   Use

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221_SLC39A14

   Plasmid

   #132105

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

   Insert

   SLC39A14 (SLC39A14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. p3xFLAG-CMV-Tlr4(Lps)

   Plasmid

   #27149

   Depositor

   Bruce Beutler

   Article

   Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

   Insert

   toll-like receptor 4(Lps) (Tlr4 Mouse)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed Proline 712 to Histidine. Native signal p…

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pFastBac-HS-NLRC4Δcard

   Plasmid

   #177276

   Purpose

   Purification of mouse NLRC4 (90-1024) from insect cells

   Depositor

   Hao Wu

   Article

   Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

   Insert

   NLRC4 (Nlrc4 Mouse)
   

   Use

   Tags

   HIS-SUMO-PreScission

   Expression

   Insect

   Mutation

   I586T, N761S, M885V, K915R and F972L- please see …

   Promoter

   polyhedrin

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEcRNAP5

   Plasmid

   #128939

   Purpose

   pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z

   Depositor

   Seth Darst

   Article

   Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

   Inserts

   RpoAppx234-241-(His)10 (rpoA Escherichia coli)
   RpoB
   RpoC
   RpoZ
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 234-241 are substituted for a PreScissio…

   Promoter

   T7

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_6xHis-PARP1-A762V

   Plasmid

   #174795

   Purpose

   Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A762V (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A762V

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 PARL-FLAG-CT H335G

    Plasmid

    #13749

    Depositor

    Luca Pellegrini

    Article

    Cipolat et al Cell. 2006 Jul 14. 126(1):163-75.

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed catalytic His 335 to Gly

    Promoter

    Available since

    Feb. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pDONR221_SLC39A5

    Plasmid

    #132057

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A5 (SLC39A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pPB_TRE3G_scFV-sfGFP-Dot1LCD-CatMut

    Plasmid

    #235591

    Purpose

    Dox-inducible expression of control catalytic-mutant Dot1l CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Dot1l (Dot1l Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    GS163-164RC

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB8709

    Plasmid

    #175224

    Purpose

    Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOL092W (CEN.PK strain)
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Dr-FGFR1

    Plasmid

    #184005

    Purpose

    Dr-FGFR expression in mammalian cells

    Depositor

    Vladislav Verkhusha

    Article

    Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

    Insert

    Dr-FGFR1 (FGFR1 Human)
    

    Use

    Tags

    Deinococcus radiodurans bacterial phytochrome

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. deltaVP1d63C + VP2C-GFPdeg

    Plasmid

    #182481

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-GFPdeg
    deltaVP1d63C
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. eDrMet

    Plasmid

    #183999

    Purpose

    Optically controlled eDrcMet expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrMet (MET Human)
    

    Use

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pESC-LEU-IpgD

    Plasmid

    #183672

    Purpose

    Inducible Shigella flexneri IpgD for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCCM’ CbbQ-

    Plasmid

    #162711

    Purpose

    Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Inactivation of CbbQ subunit of CbbOQ rubisco act…

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCCM’ ΔacRAF

    Plasmid

    #162710

    Purpose

    Second CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAF

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    pCCM' with a deletion of putative rubisco ch…

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His

    Plasmid

    #83458

    Purpose

    Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Amino-acids 4127-4303, S4144D

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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