We narrowed to 5,457 results for: golden gate
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Plasmid#217604PurposeDestination vector with ampicillin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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DVC_p15A_AF
Plasmid#217588PurposeDestination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1047
Plasmid#222726PurposepNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
TagssfGFPExpressionBacterialAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_NANOS3_cterm
Plasmid#222904PurposeCas9/sgRNA plasmid for targeting NANOS3DepositorPublicationInsertCas9, NANOS3 sgRNA (NANOS3 Human, Synthetic)
UseCRISPRAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv4
Plasmid#221432PurposeLentiviral CRISPR/Cas9 vector for co-expression of sgRNAs and Cas9 protein.DepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNK2657
Plasmid#219741PurposeMoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiensDepositorInserthispidin- synthase from Mycena citricolor
UseSynthetic BiologyAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK5712
Plasmid#219752PurposepGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnH3H_v2 - tAOX
ExpressionYeastAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT6471
Plasmid#219428PurposeModule of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)DepositorInsertAGGT_NLS-SpCas9i-NLS(no stop)_TTCG
UseMoclo compatible level 0 moduleAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-SRSF1 siRNA-resitant
Plasmid#218974PurposeVector for expressing siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGGAGUUUGUACGGAAA)DepositorInsertSRSF1 cDNA
ExpressionMammalianMutationsiRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGG…PromoterCMVAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT9050
Plasmid#219429PurposePapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)DepositorInsertAATG_PapE-4xLF2_AGGT
UseMoclo compatible level 0 moduleAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-pT231 scFv-GFP
Plasmid#218827PurposeYeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFPDepositorInsertpT231 tau scFv
ExpressionYeastPromoterGAL1-10Available sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G555
Plasmid#216483PurposeExpression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertplsB, plsC, cdsA, pssA
UseCell-free expressionPromoterT7 for each geneAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH-chl-roGFP2-ORP1
Plasmid#191699PurposeTi plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells.DepositorInsertchl-roGFP2-ORP1
UseBinary agrobacterium ti plasmidExpressionPlantPromoterCaMV 35S promoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPD057 VP64-ZF37
Plasmid#138834PurposeConstitutive expression of VP64-ZF37 under the CMV promoter.DepositorInsertVP64-ZF37
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD536 VP16-ZF150
Plasmid#138769PurposeConstitutive expression of VP16-ZF150 under the CMV promoter.DepositorInsertVP16-ZF150
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD538 VP16-ZF172
Plasmid#138772PurposeConstitutive expression of VP16-ZF172 under the CMV promoter.DepositorInsertVP16-ZF172
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise
Plasmid#129650PurposeRieske FeS overexpressionDepositorInsertCDS of PetC gene encoding for the Rieske FeS subunit
ExpressionPlantMutationCodon modified for Golden GatePromoterpZmUbiAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL009
Plasmid#122862PurposeBinary expression vector containing 35S:MS2-SRDX:tRBCS followed by GreenGate A-G sequences for insertion of additional modulesDepositorInsert35S:MS2-SRDX:tRBCS
UseGolden gate compatible plant transformation vectorAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDG058
Plasmid#122840PurposeSRDX repressor domain with GreenGate D-E flanking sequencesDepositorInsertSRDX transcriptional repressor domain
UseGolden gate compatible cloning vectorAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH185
Plasmid#122839PurposeVP64 activator domain with GreenGate D-E flanking sequencesDepositorInsertVP64 transcriptional activator domain
UseGolden gate compatible cloning vectorAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only