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We narrowed to 5,541 results for: pcas

Showing: 2461 - 2480 of 5541 results

  3. pCASCADE-fabI-gltA1-gltA2-udhA-zwf

    Plasmid
    #87148
    Purpose
    fabI-gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-udhA-zwf guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87148-plasmid-map-sequence-id-175306

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  4. pCASCADE-fabI-gltA1-gltA2-udhA-gapAP1

    Plasmid
    #87154
    Purpose
    fabI-gltA1-gltA2-udhA-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-udhA-GapAP1 guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87154-plasmid-map-sequence-id-174942

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  7. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    103865-plasmid-map-sequence-id-243540

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  8. AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)

    Plasmid
    #197509
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Expression
    Mutation
    ABE8e mutations in TadA and nSpCas9(D10A)
    Promoter
    Cbh
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197509-plasmid-map-sequence-id-391242

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  9. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

    Plasmid
    #223113
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223113-plasmid-map-sequence-id-447636

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  10. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid
    #197502
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197502-plasmid-map-sequence-id-386612

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  11. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid
    #185913
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185913-plasmid-map-sequence-id-367365

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  14. pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

    Plasmid
    #223114
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223114-plasmid-map-sequence-id-447638

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  16. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid
    #140005
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140005-plasmid-map-sequence-id-275488

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  20. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid
    #225756
    Purpose
    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection
    Depositor
    Paul Blainey
    Article
    Walton et al bioRxiv 2024.03.17.585235
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225756-plasmid-map-sequence-id-446064

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Showing: 2461 - 2480 of 5541 results