We narrowed to 54,445 results for: plasmid

1. AiP14534 - pAAV-AiE0390m_3xC2-minBglobin-iAChSnFR-Venus-WPRE3-BGHpA (Alias: CN4534)

   Plasmid

   #208164

   Purpose

   Direct-expressing iAChSnFR-Venus in astrocytes

   Depositor

   Allen Institute for Brain Science, Boaz Levi

   Insert

   iAChSnFR-Venus
   

   Use

   AAV

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Q-IL6-9

   Plasmid

   #166552

   Purpose

   scFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptide

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, Sortag, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. P48

   Plasmid

   #212155

   Purpose

   Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.

   Depositor

   Kang Zhou

   Insert

   superfolder green fluorescent protein, secondary alcohol dehydrogenase
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   PgyrA

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWPI-IRES-Bla-Ak-ACE2

   Plasmid

   #154981

   Purpose

   Lentiviral vector expressing Human ACE2

   Depositor

   Sonja Best

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P1 METTL3

   Plasmid

   #200731

   Purpose

   Bacterial expression of GST-Tagged codon optimized human METTL3

   Depositor

   Claudio Alarcón

   Insert

   METTL3 (METTL3 Human)
   

   Tags

   GST from backbone plasmid

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. IMBB2.4-pUC57-mini

   Plasmid

   #88845

   Purpose

   plasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2017 Mar 1;62(3):99-106.

   Type

   Empty backbone

   Use

   Synthetic Biology; Cloning

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SIRT6

   Plasmid

   #41564

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SIRT6 (SIRT6 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   aa 3-318

   Promoter

   T7

   Available Since

   April 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

8. T-IL2-12

   Plasmid

   #166564

   Purpose

   scFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptide

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, Sortag, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Q-IL18-6

   Plasmid

   #166554

   Purpose

   scFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptide

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, Sortag, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.2-V5-DEST-hSFRP1-VF

    Plasmid

    #189542

    Purpose

    Protein expression of SFRP1 in mammalian cells.

    Depositor

    Haian Fu

    Insert

    secreted frizzled related protein 1 (SFRP1 Human)
    

    Tags

    VF

    Expression

    Mammalian

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMB52 / Human_LRRK1_1_360

    Plasmid

    #40549

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK1 (LRRK1 Human)
    

    Use

    Baculovirus

    Tags

    6xHis

    Mutation

    WT

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV hSyn-Flex RG-cerulean

    Plasmid

    #98221

    Purpose

    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus

    Depositor

    Troy Margrie

    Insert

    Cerulean-E2A-RG
    

    Use

    AAV

    Tags

    cerulean

    Promoter

    human Synapsin

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. SDCBPA-c000

    Plasmid

    #175480

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-V288.

    Depositor

    Opher Gileadi

    Insert

    SDCBP (SDCBP Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUPD2 PhyB (GB2721)

    Plasmid

    #160603

    Purpose

    chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.

    Depositor

    Diego Orzaez

    Insert

    PhyB
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Z-AIMP1-1

    Plasmid

    #166560

    Purpose

    scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV Agpat2-V5

    Plasmid

    #175159

    Purpose

    Lentiviral expression of mouse Agpat2-V5

    Depositor

    Larry Gerace

    Insert

    Agpat2 (Agpat2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. G-BRD4-4

    Plasmid

    #166518

    Purpose

    scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 44-168 of 1362, Bromodomain 1.

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. APEX2 VLP

    Plasmid

    #182211

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).

    Depositor

    Claudia Vickers

    Inserts

    VP2C-APEX2
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology; Tem imaging

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV Cnep1r1-V5

    Plasmid

    #175133

    Purpose

    Lentiviral expression of mouse Cnep1r1-V5

    Depositor

    Larry Gerace

    Insert

    Cnep1r1 (Cnep1r1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV Ef1a-mRuby3-WPRE

    Plasmid

    #135427

    Purpose

    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

    Depositor

    Rylan Larsen

    Insert

    mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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