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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

Plasmid

#129029

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA1 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-bio-myc-mCE 2-210

Plasmid

#112715

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBKS_WT_V5

Plasmid

#82959

Purpose

Gateway Donor vector containing RBKS, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBKS (RBKS Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAP2_WT_V5

Plasmid

#83000

Purpose

Gateway Donor vector containing STAP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STAP2 (STAP2 Human)

Use

Gateway Cloning

Mutation

D93N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPOX_WT_V5

Plasmid

#82951

Purpose

Gateway Donor vector containing PPOX, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PPOX (PPOX Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAB31_WT_V5

Plasmid

#82993

Purpose

Gateway Donor vector containing RAB31, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RAB31 (RAB31 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT3-T2A-Cre

Plasmid

#189008

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT3 transcript variant 1 ORF and Cre recombinase, separated by T2A motifs

Depositor

Bryan Luikart

Article

Prina et al Brain. 2026 Mar 9:awag098. doi: 10.1093/

Insert

AKT3 (AKT3 Human)

Use

Retroviral

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT1-T2A-Cre

Plasmid

#189011

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT1 ORF, and Cre recombinase, separated by T2A motifs.

Depositor

Bryan Luikart

Article

Prina et al Brain. 2026 Mar 9:awag098. doi: 10.1093/

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAP-Tag5-IgKsp-mHevin∆DE-Myc-His

Plasmid

#249177

Purpose

Overexpression of mouse Sparcl1 with deletion of a.a. 330-550 with Myc and 6xHis tag

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Deleted amino acids 330–550

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-mDE-Myc-His

Plasmid

#249180

Purpose

AAV mediated overexpression of mouse Sparcl1 a.a. 330-550 fragment

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Use

AAV

Tags

6xHis and Myc

Expression

Mammalian

Mutation

amino acids 330–550

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pSA075 Cas9-BFP-HBB_IVS2

Plasmid

#249136

Purpose

Overexpression of SpCas9-BFP with HBB IVS2 intron in 3' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

Cas9-TagBFP-HBB_IVS2 (HBB S. pyogenes Cas9, synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

HBB IVS2 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA072 Cas9-HBB_IVS2-BFP

Plasmid

#249133

Purpose

Overexpression of SpCas9-BFP with HBB IVS2 intron after Cas9 coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

Cas9-HBB_IVS2-TagBFP (HBB S. pyogenes Cas9, synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

HBB IVS2 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA096 PSMD4_IVS6-Cas9-BFP

Plasmid

#249146

Purpose

Overexpression of SpCas9-BFP with PSMD4 IVS6 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

PSMD4_IVS6-Cas9-TagBFP (PSMD4 S. pyogenes Cas9, synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

PSMD4 IVS6 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPB-shDrp1-mCherry-CAAX

Plasmid

#228739

Purpose

Membrane-tethered mCherry shRNA targeting Drp1

Depositor

Cagla Eroglu

Article

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Drp1 (Dnm1l, Dnm1l Mouse, Rat)

Expression

Mammalian

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shDrp1-GFP

Plasmid

#228737

Purpose

GFP expressing shRNA targeting Drp1

Depositor

Cagla Eroglu

Article

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Drp1 (Dnm1l, Dnm1l Mouse, Rat)

Expression

Mammalian

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pvPE-V3-Puro

Plasmid

#240289

Purpose

Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene in to mammalian cells for gene editing.

Depositor

Lusheng Huang

Article

Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

Insert

pvPE-V3-Puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1

Plasmid

#196879

Purpose

Neuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mOrange2-rCM1 (Nedd1 Rat)

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Akna-mOrange2

Plasmid

#196865

Purpose

Expression of the centrosomal protein Akna fused to mOrange2

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akna-mOrange2 (Akna Mouse)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV Cdk2ap1ΔN (MT2B2)-HA

Plasmid

#178031

Purpose

Retroviral vector for the purpose of overexpression in mammalian cell culture

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

First N-Terminal 27aa are deleted

Promoter

GAG

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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I282L

Plasmid

#184308

Purpose

To study dynamics of G-actin using NMR spectroscopy.

Depositor

Michael Rosen

Article

Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

Insert

Act5C (Act5C Fly)

Tags

B-Thymosin followedby hexahistidine tag

Expression

Yeast

Mutation

I282L

Promoter

AOX1

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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