We narrowed to 6,879 results for: crispr cas9 plasmids

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pBK1313-AAV-EFSNC-dSaCas9-MECP2(204-310)

Plasmid

#223158

Purpose

Expression of truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

Plasmid

#223155

Purpose

Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD2 (MBD2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 176-231

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

Plasmid

#223149

Purpose

Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

Plasmid

#165450

Purpose

Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

Depositor

Elvir Becirovic

Article

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Inserts

dCas9N-IntN
3x Opn1mw promoter-targeting sgRNAs

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6 and short human rhodopsin promoter

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

Plasmid

#223159

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

Plasmid

#209781

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

cTNT

Use

AAV

Tags

HA and OLLAS

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1297-AAV-EFSNC-dCjCas9-HP1b(79-173)

Plasmid

#223142

Purpose

Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 79-173

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)

Plasmid

#223152

Purpose

Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 79-173

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9

Plasmid

#190903

Purpose

AAV-KamiCas9 vector expressing expressing thew GFP reporter gene and sgHTT and sgCas9

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

GFP, sgHTT and sgCas9 (HTT Human)

Use

AAV and CRISPR

Promoter

EFS and U6

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

Plasmid

#166133

Purpose

This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

Depositor

Raphael Gaudin

Article

Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

Insert

hU6-gRNA and hH1-gRNA targeting cldn1 gene

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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