We narrowed to 9,324 results for: bli

1. pDON-AI-baIaa

   Plasmid

   #51474

   Purpose

   Express mouse TH2B and TH2A in mammalian cells

   Depositor

   Shunsuke Ishii

   Inserts

   histone cluster 1, H2aa (H2ac1 Mouse)
   histone cluster 1, H2ba (H2bc1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTF280 (pFA6a-PSc-6xGly-2xStrep-HphMX)

   Plasmid

   #44076

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   2xStrep tag, 6xGly linker, ADH1 transcription ter…

   Available Since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lazarus Gata 3 flag

   Plasmid

   #80135

   Purpose

   retroviral expression of Gata3-Flag

   Depositor

   Ellen Rothenberg

   Insert

   GATA binding protein 3 (Gata3 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG and IRES-EGFP

   Expression

   Mammalian

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. gamma1-EGFP

   Plasmid

   #119224

   Purpose

   GABAA receptor expression (gamma 1 subunit with EGFP tag at the C-terminus)

   Depositor

   Angel de Blas

   Insert

   GABRG1 (Gabrg1 Rat)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUASp YFP Cdc42 CA

   Plasmid

   #52250

   Purpose

   Drosophila expression of YFP-Cdc42 constitutively active mutant

   Depositor

   Matthew Scott

   Insert

   Cdc42 (Cdc42 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q61L; CA mutation

   Promoter

   GAL4

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

6. PTPRO

   Plasmid

   #38969

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PTPRO (PTPRO Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pPyCAG-Nanog(L122A)-IP

   Plasmid

   #62992

   Purpose

   Expresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog L122A Mutant (Nanog Mouse)
   

   Expression

   Mammalian

   Mutation

   Changed Leucine 122 (123 AA in Mouse Protein) to …

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. mouse pGL3-ank3_1-promoter firefly luciferase

   Plasmid

   #86436

   Purpose

   Mouse pGL3-ank3_1-promoter firefly luciferase, ca 2000 base pairs around TSS exon 1e

   Depositor

   Hans-Georg Koenig, Jochen Prehn

   Insert

   ank3_1 promoter
   

   Tags

   n/a

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   ankyrin-3_1

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. HaloTag-G protein gamma-10

   Plasmid

   #67041

   Purpose

   G protein gamma-10 tagged with HaloTag on amino terminus

   Depositor

   Catherine Berlot

   Insert

   HaloTag-G protein gamma-10 (GNG10 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKG194

    Plasmid

    #69757

    Purpose

    Mammalian Expression of CENP-T aa 1-375

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-375 (CENPT Human)
    

    Use

    Retroviral

    Tags

    LacI-NLS and eGFP

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Available Since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

11. GYG1

    Plasmid

    #39091

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GYG1 (GYS1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. COVID-SARS2 NSP10

    Plasmid

    #159616

    Purpose

    Bacterial expression plasmid for COVID-SARS2 NSP10 fused to GST

    Depositor

    Opher Gileadi

    Insert

    Covid-SARS2 Nsp10
    

    Tags

    GST-3C protease site

    Expression

    Bacterial

    Promoter

    T7 - LacO

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. FL-DYSF-pUC57

    Plasmid

    #60177

    Purpose

    Plasmid contains full-length human dysferlin in a multiple cloning site of pUC57-Kan and can be easily cloned into other vectors.

    Depositor

    Jain Foundation

    Insert

    Dysferlin (DYSF Human)
    

    Use

    Cloning vector

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. RFXANK (3UXG)

    Plasmid

    #36902

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RFXANK (RFXANK Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pIC146

    Plasmid

    #63069

    Purpose

    Retroviral expression of EGFP-TEV-S-tag CENP-P for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    CENP-P, IMAGE clone 5554572 (CENPP Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. PS-CFP2-Alpha-5-Integrin-12

    Plasmid

    #62794

    Purpose

    Localization: Focal Adhesions, Excitation:400 / 490, Emission:468 / 511

    Depositor

    Michael Davidson

    Insert

    Alpha5Integrin (ITGAV Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. CBR3

    Plasmid

    #38800

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CBR3 (Cbr3 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

18. CA13

    Plasmid

    #38924

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CA13 (CA13 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pLV Lnpk-V5

    Plasmid

    #175121

    Purpose

    Lentiviral expression of mouse Lnpk-V5

    Depositor

    Larry Gerace

    Insert

    Lnpk (Lnpk Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV V5-Sun1

    Plasmid

    #175113

    Purpose

    Lentiviral expression of V5-tagged mouse Sun1

    Depositor

    Larry Gerace

    Insert

    Sun1 (Sun1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. KSP_K406

    Plasmid

    #129771

    Purpose

    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays

    Depositor

    William Hancock

    Insert

    KSP (kif11.L Frog)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Truncation at 367, fused to kinesin-1 starting Al…

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pUAST-FOXO WT

    Plasmid

    #52982

    Purpose

    insect expression of FOXO WT

    Depositor

    Biao Wang, Marc Montminy

    Insert

    FOXO (foxo Fly)
    

    Tags

    HA

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pTF269 (pFA6a-PSc-6xGly-12xHis-KanMX)

    Plasmid

    #44087

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    12xHis tag, 6xGly linker, ADH1 transcription term…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZC08 (pFA6a-PSc-6xGly-PrtA-HphMX)

    Plasmid

    #44078

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, HphM…

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTF278 (pFA6a-PSc-6xGly-2xStrep-KanMX)

    Plasmid

    #44088

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    2xStrep tag, 6xGly linker, ADH1 transcription ter…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)

    Plasmid

    #44075

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    12xHis tag, 6xGly linker, ADH1 transcription term…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hKv2.1-DN-HA

    Plasmid

    #111530

    Purpose

    Expresses a non-conducting dominant neg in mammalian cells

    Depositor

    Federico Sesti

    Insert

    human W369C-Y384T-Kv2.1 + HA (KCNB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    W369C-Y384T

    Promoter

    CMV

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pJK276

    Plasmid

    #73366

    Purpose

    Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)

    Depositor

    T. J. Kappock

    Insert

    plastid omega-3 fatty acid desaturase
    

    Expression

    Yeast

    Mutation

    Mutation I231F. Also insert is missing residues 1…

    Promoter

    GAL1

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. p-dCas9-SET7-Hygro

    Plasmid

    #104407

    Purpose

    transient expression of dCas9-SET7 fusion protein

    Depositor

    Stephan Beck

    Insert

    SET7 (SETD7 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCHFLAGGR

    Plasmid

    #1279

    Depositor

    Susan Lindquist

    Article

    Kimura et al Science 1995 Jun 2;268(5215):1362-5.

    Insert

    GR (Nr3c1 Rat)
    

    Tags

    FLAG

    Expression

    Yeast

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

31. SOCS6

    Plasmid

    #38871

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SOCS6 (SOCS6 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    V368D, R463G

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

32. USP37

    Plasmid

    #36898

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP37 (USP37 Human)
    

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 4-125 only

    Available Since

    June 21, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

33. pZC04 (pFA6a-PSc-6xGly-PrtA-His3MX)

    Plasmid

    #44066

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, His3…

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHH0103 APBB3 WW domain

    Plasmid

    #104262

    Purpose

    Bacterial expression of WW domain from APBB3

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    APBB3 WW domain (APBB3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHH0103 APBB2 WW domain

    Plasmid

    #104257

    Purpose

    Bacterial expression of WW domain from APBB2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    APBB2 WW domain (APBB2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTF276 (pFA6a-PSc-6xGly-2xStrep-His3MX)

    Plasmid

    #44064

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    2xStrep tag, 6xGly linker, ADH1 transcription ter…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUASp YFP Rholike DN

    Plasmid

    #52240

    Purpose

    Drosophila expression of YFP-Rholike dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Rholike (RhoL Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T25N; DN mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pAc.1B-EGFP-DmHrb98

    Plasmid

    #66595

    Purpose

    Expresses EGFP-tagged Hrb98 in Drosophila cells

    Depositor

    Elisa Izaurralde

    Insert

    Hrb98 (Hrb98DE Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Promoter

    Actin 5C

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCRII-Topo Lhx2

    Plasmid

    #45873

    Depositor

    Jeffrey Macklis

    Insert

    Lhx2 in situ probe (Lhx2 Mouse)
    

    Use

    In situ

    Mutation

    contains bp# 1809-2217 of NM_010710.3

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pJK693

    Plasmid

    #72382

    Purpose

    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)

    Depositor

    T. J. Kappock

    Insert

    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutated alanine-260 to threonine

    Promoter

    T7

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge