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We narrowed to 5,034 results for: plasmid dna

Showing: 4961 - 5000 of 5034 results

  1. MAP3K15-TAP

    Plasmid
    #69728
    Purpose
    Express tandem tagged MAP3K15 in cells
    Depositor
    Daniel Liebler
    Insert
    Apoptosis signal-regulating kinase 3 (MAP3K15 Human)
    Use
    Tags
    HA-Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    69728-plasmid-map-sequence-id-128183

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  2. MAP3K5-TAP

    Plasmid
    #69726
    Purpose
    Express tandem tagged MAP3K5 in cells
    Depositor
    Daniel Liebler
    Insert
    Apoptosis signal-regulating kinase 1 (MAP3K5 Human)
    Use
    Tags
    HA-FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    69726-plasmid-map-sequence-id-123455

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  4. anti-VGluT1 scFv [N28/9] with sortase tag

    Plasmid
    #204424
    Purpose
    Mammalian Expression of anti-VGluT1 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N28/9.
    Depositor
    Jeff Lichtman
    Article
    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d
    Insert
    Recombinant mouse scFv targeting VGluT1 (Rattus norvegicus)
    Use
    Tags
    6xHis tag and Sortase tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204424-plasmid-map-sequence-id-406613

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  7. anti-Calbindin scFv [L109/57] with sortase tag

    Plasmid
    #204421
    Purpose
    Mammalian Expression of anti-Calbindin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L109/57.
    Depositor
    Jeff Lichtman
    Article
    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d
    Insert
    Recombinant mouse scFv targeting Calbindin (Homo sapiens)
    Use
    Tags
    6xHis tag and Sortase tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204421-plasmid-map-sequence-id-406615

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  8. anti-Parvalbumin scFv [L114/81] with sortase tag

    Plasmid
    #204423
    Purpose
    Mammalian Expression of anti-Parvalbumin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L114/81.
    Depositor
    Jeff Lichtman
    Article
    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d
    Insert
    Recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus)
    Use
    Tags
    6xHis tag and Sortase tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204423-plasmid-map-sequence-id-406609

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  10. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid
    #162576
    Purpose
    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.
    Depositor
    George Church
    Article
    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038
    Inserts
    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K mutated to give dominant negative phenotype
    Promoter
    pBAD
    Available since
    May 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162576-plasmid-map-sequence-id-326400

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  11. anti-NPY/Neuropeptide Y scFv [L115/13] with sortase tag

    Plasmid
    #204426
    Purpose
    Mammalian Expression of anti-Neuropeptide Y scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L115/13.
    Depositor
    Jeff Lichtman
    Article
    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d
    Insert
    Recombinant mouse scFv targeting NPY/Neuropeptide Y (Homo sapiens)
    Use
    Tags
    6xHis tag and Sortase tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204426-plasmid-map-sequence-id-406621

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  12. anti-GFAP R416WT scFv [N206B/9] with sortase tag

    Plasmid
    #204420
    Purpose
    Mammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.
    Depositor
    Jeff Lichtman
    Article
    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d
    Insert
    Recombinant mouse scFv targeting GFAP R416WT (Homo sapiens)
    Use
    Tags
    6xHis tag and Sortase tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204420-plasmid-map-sequence-id-406611

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  18. SpyTag-Alternate Quartet

    Plasmid
    #214728
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses pang17, RmYN02, Rf1, and WIV1 each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Alternate Quartet
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214728-plasmid-map-sequence-id-435533

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  19. SpyTag-Quartet [SARS1]

    Plasmid
    #214729
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Quartet [SARS1] (S Synthetic)
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214729-plasmid-map-sequence-id-435534

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  20. pLenti-nSpCas9-APOBEC3A

    Plasmid
    #209040
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC3A-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209040-plasmid-map-sequence-id-413592

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  24. GluN2B/NR2B glutamate receptor scFv [N59/36]

    Plasmid
    #182093
    Purpose
    Mammalian Expression of GluN2B/NR2B glutamate receptor scFv. Derived from hybridoma N59/36.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    recombinant mouse scFv targeting GluN2B/NR2B glutamate receptor (Rattus norvegicus) (Grin2b Mouse)
    Use
    Tags
    HA, His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    182093-plasmid-map-sequence-id-357628

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  25. GluN2B/NR2B glutamate receptor scFv [N59/20]

    Plasmid
    #182092
    Purpose
    Mammalian Expression of GluN2B/NR2B glutamate receptor scFv. Derived from hybridoma N59/20.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    recombinant mouse scFv targeting GluN2B/NR2B glutamate receptor (Rattus norvegicus) (Grin2b Mouse)
    Use
    Tags
    Sortase, His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    182092-plasmid-map-sequence-id-357627

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  31. pLenti-nSpCas9-APOBEC1

    Plasmid
    #209039
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209039-plasmid-map-sequence-id-413619

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  32. pLenti-nSpCas9-BEACON2

    Plasmid
    #209041
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    BEACON2-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209041-plasmid-map-sequence-id-419488

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  33. pLenti-nSpCas9-evoCDA1

    Plasmid
    #209042
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    evoCDA1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209042-plasmid-map-sequence-id-413615

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  37. lucMAPT-30D

    Plasmid
    #214672
    Purpose
    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Use
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9, Exon 10 with a mutation to intro…
    Promoter
    CMV
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214672-plasmid-map-sequence-id-424908

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  38. lucMAPT-30U

    Plasmid
    #214673
    Purpose
    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Use
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9, Exon 10 with a mutation to intro…
    Promoter
    CMV
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214673-plasmid-map-sequence-id-424933

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  39. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

    Plasmid
    #185706
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS
    Depositor
    Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    Cas9
    Use
    Tags
    6xHis-NLS and NLS
    Expression
    Mammalian
    Mutation
    optimized for human codon usage
    Promoter
    CMV and T7
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185706-plasmid-map-sequence-id-363635

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Showing: 4961 - 5000 of 5034 results