We narrowed to 7,396 results for: tet on
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Plasmid#214751PurposeTo create delta(glmS,nadE) working strains and maintaining their viabilityDepositorInsertBBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
UseCRISPRExpressionBacterialAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKF-P14MM2AG5h
Plasmid#128254PurposeMammalian positive feedback synthetic gene circuit within a Flp-In expression vector.DepositorInsertrtTA-Advanced::P2A::EGFP
UseSynthetic Biology; Flp-in expression vectorExpressionMammalianPromotertight TRE promoterAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWKS1583
Plasmid#70188PurposeModular vector for secreted amylase reporter expressionDepositorInsertamyEopt
UseSynthetic BiologyExpressionBacterialPromoterPtetAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRTS-GBP-dCas9-mRFP
Plasmid#102934PurposeTetracyclin inducible expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)DepositorInsertdCas9
UseCRISPRTagsGBP (GFP binding protein and mRFPExpressionMammalianAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5
Plasmid#64985PurposeExpresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZH519
Plasmid#102672PurposeTetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV+ plasmid with onboard mCherry cassette
Plasmid#169741PurposeCMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyExpressionMammalianPromoterpCMV-tetO2Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
JAB2098
Plasmid#190340PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt
ExpressionPlantAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pV2A-T
Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZH518
Plasmid#102671PurposeTetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
prCAG-RBS"m1"-sfGFP
Plasmid#191400PurposeExpression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1)DepositorInsert47xCAG RBS"m1"-sfGFP
UseSynthetic BiologyExpressionBacterialPromoterPLtet0-1Available SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R400
Plasmid#138175PurposeExpresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHTIKA
Plasmid#22062DepositorInserthCMV-TetOn expression cassette
ExpressionMammalianAvailable SinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLLX13
Plasmid#79825PurposeYeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vectorDepositorTypeEmpty backboneUseYeast and bacteria shuttle vector, contains origi…Available SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSecEffector
Plasmid#172891PurposeContains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901DepositorInsertsBxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites
ExpressionBacterialPromoterProD, nTETO, and pBAD /AraCAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA7woH
Plasmid#65020PurposeExpresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, E105K, E108A, D16…Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZH509
Plasmid#102664PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::tTA2-bGHpA
Plasmid#62447PurposeMXS_chaining vector with CMV::tTA2-bGHpADepositorInsertcassette containing the tetracycline transactivator with CMV promoter
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132D2.0
Plasmid#99890PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU3Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA-sbtA
Plasmid#133011PurposepFA carrying a Synechococcus elongatus PCC 7942 sbtA gene (Uniprot: Q6UP27)DepositorInsertSbtA
Tags6xhisExpressionBacterialPromotertetRAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRJPaph-eBFP2
Plasmid#67030PurposePlasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
UseConjugative bacterial vectorAvailable SinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDM8-GlyCAM-1/IgG
Plasmid#17841DepositorInsertGlyCAM-1/IgG (Glycam1 Mouse)
ExpressionMammalianAvailable SinceMarch 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pZA-mCherry-R5
Plasmid#106258PurposeExpresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertmCherry
TagsAdded silaffin R5 peptide to C terminus of mCherr…ExpressionBacterialAvailable SinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB577
Plasmid#83778PurposePhenylalanine biosensor plasmidDepositorInsertpromoter of RL1860
Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R375
Plasmid#138245PurposeExpresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZH512
Plasmid#102666PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLMB686
Plasmid#83780Purposemyo-Inositol biosensor plasmidDepositorInsertupstream region of RL4655 (intA)
Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EC0_2_VP64
Plasmid#163714PurposePlasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)DepositorInsertVP64 + SV40 NLS
UseSynthetic Biology; Moclo vector level 0 (type 4a)Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB237
Plasmid#170030PurposeEncodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (2)
Plasmid#185852PurposeTesting the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
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HL 818 (=HL 716 + pHL 98)
Plasmid#30006DepositorInsertpLlacO-1:DsrA-mcherry, pLtetO-1:rpoS::gfp
UseSynthetic BiologyTagsmcherry, GFPExpressionBacterialAvailable SinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLMB684
Plasmid#83779PurposeXylose biosensor plasmidDepositorInsertupstream region of RL2720 (rbsC)
Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH511
Plasmid#102665PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP.R271
Plasmid#138220PurposeExpresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLMB687
Plasmid#83781PurposeSucrose biosensor plasmidDepositorInsertRegion upstream pRL120556 containing all of pRL120555
Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133D2.0
Plasmid#99894PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU3Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131D2.0
Plasmid#100044PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU3Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
AEB-TMR10pUC19
Plasmid#63858PurposeThis pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene.DepositorInsertTMR10 TMR riboswitch
UseSynthetic BiologyPromotertacAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA-eCA
Plasmid#133010PurposepFA carrying a E. coli canA gene (Uniprot: P61517)DepositorAvailable SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT241_(pCA-ATG-intron-tTA2)
Plasmid#36875DepositorInserttTA2
ExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available SinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSG 413
Plasmid#63859PurposeDetect translation initiation from CUG start codon (E. coli lacZ)DepositorAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFP.R365
Plasmid#138235PurposeExpresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-SwiChR-eYFP
Plasmid#166604PurposeEncodes Cre-dependent SwiChR-eYFP under control of the TREDepositorInsertSwiChR-eYFP
UseAAVPromotertetracycline response elementAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLMB708
Plasmid#83782PurposeTartrate biosensor plasmidDepositorInsertRegion upstream RL0996 containing all of RL0997
Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB710
Plasmid#83783PurposeFormate biosensor plasmidDepositorInsertRegion upstream RL4393 (fdsG) containing all of RL4394
Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB712
Plasmid#83784PurposeHesperetin biosensor plasmidDepositorInsertRegion upstream pRL100185 (nodA) containing part, but not all of, pRL100184 (nodD)
Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB714
Plasmid#83786PurposeC4-dicarboxylates biosensor plasmidDepositorInsertRegion upstream RL3424 (dctA)
Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB705
Plasmid#83787PurposeMannitol biosensor plasmidDepositorInsertRegion upstream RL4218 (mtlB) containing all of RL4219
Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB717
Plasmid#83788PurposeErythritol biosensor plasmidDepositorInsertRegion upstream pRL90085 containing all of pRL90087
Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only