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pSB115 - pL2_pSB90_fLUC-I_tGFP-I

Plasmid

#123190

Purpose

binary plant vector for transient tGFP (with intron) and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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221-RPB1-AID-eGFP-2A-blasticidin

Plasmid

#195107

Purpose

Targeting vector to introduce AID-eGFP tags in C-terminal of the human RPB1 gene. Blasticidin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

AID-eGFP

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-OsMIR390-B/c

Plasmid

#61468

Purpose

Gateway-compatible entry vector for direct cloning of artificial microRNAs into rice MIR390 precursor.

Depositor

James Carrington

Article

Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

Insert

OsMIR390-B/c

Use

Gateway entry vector

Mutation

O. sativa MIR390 precursor sequence including a c…

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pFAB822

Plasmid

#47856

Purpose

BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

BBa_B1006 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO11

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB803

Plasmid

#47847

Purpose

BIOFAB reporter plasmid for measuring T7 early termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

T7 early terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO2

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS

Plasmid

#123188

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDN-T2dGZmxh

Plasmid

#44552

Depositor

Gabor Balazsi

Article

Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

Inserts

PTETREG promoter
yEGFP::ZeoR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Promoter

PTETREG

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pGL2-HKII-A

Plasmid

#64513

Purpose

AS-30D hepatoma hexokinase II 4.3 kbp promoter-luciferase reporter vector

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

Insert

Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)

Use

Luciferase

Tags

firefly luciferase

Mutation

Isolated from AS-30D rat hepatoma

Promoter

Hexokinase II

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pGL2-HKII-C

Plasmid

#64515

Purpose

AS-30D hepatoma hexokinase II 2.1 kbp promoter-luciferase reporter vector

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

Insert

Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)

Use

Luciferase

Tags

firefly luciferase

Mutation

Isolated from AS-30D rat hepatoma

Promoter

Hexokinase II

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
pGL2-HKII-E

Plasmid

#64517

Purpose

AS-30D hepatoma hexokinase II 0.1 kbp promoter-luciferase reporter vector

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

Insert

Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)

Use

Luciferase

Tags

firefly luciferase

Mutation

Isolated from AS-30D rat hepatoma

Promoter

Hexokinase II

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Btg1

Plasmid

#45865

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Btg1 in situ probe (Btg1 Mouse)

Use

In situ

Mutation

contains bp# 1004-1638 of BC006834.1

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pFAB3906

Plasmid

#47809

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD2 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD2

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pVT-Ruby

Plasmid

#234918

Purpose

This all-in-one vector is used to express the Ruby gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

Ruby

Expression

Plant

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLX-TRV2-vCHLI

Plasmid

#239842

Purpose

Derivative of pLX-TRV2, a tobacco rattle virus (TRV) vector, designed for the expression of a 32-nt insert to silence CHLI homolog genes in Nicotiana benthamiana and Solanum aethiopicum

Depositor

Fabio Pasin

Article

Garcia et al Plant Biotechnol J. 2025 Nov;23(11):4930

Insert

vCHLI

Use

RNAi

Expression

Plant

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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Amber-free pNL4-3 FLuc Env-RT-

Plasmid

#238415

Purpose

An amber-free HIV NL4-3 carrying a Firefly luciferase reporter gene, RT and Env impaired.

Depositor

Maolin Lu

Article

Xu et al STAR Protoc. 2025 Jul 26;6(3):103995. do

Insert

Firefly luciferase (LOC116160065 Synthetic)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Alternation of amber stop codons "TAG" …

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW10K_0x5

Plasmid

#177278

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVO-NanoLUC

Plasmid

#234900

Purpose

This all-in-one vector is used to overexpress the NanoLUC gene via virus-mediated overexpression (VOX)

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

NanoLUC

Expression

Plant

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pVT-LUC

Plasmid

#234915

Purpose

This all-in-one vector is used to express the LUC gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

LUC

Expression

Plant

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235268

Purpose

Lentiviral expression of ComMAND base gene regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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SRGAP2C_pEF-DEST51

Plasmid

#238497

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

SRGAP2C (SRGAP2C Human)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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