We narrowed to 3,663 results for: golden gate

1. pXTK003 [Marker_GenR-aacC1_PartType2]

   Plasmid

   #229240

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_GenR-aacC1_PartType2

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   GenR-aacC1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pXTK006 [Marker_CamR-cat_PartType2]

   Plasmid

   #229243

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_CamR-cat_PartType2

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   CamR-cat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pXTK009 [Marker_TetR-tetAR_PartType4]

   Plasmid

   #229246

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_TetR-tetAR_PartType4

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   TetR-tetAR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXTK010 [Marker_GenR-aacC1_PartType4]

   Plasmid

   #229247

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_GenR-aacC1_PartType4

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   GenR-aacC1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Gal4-pcVP16-1

   Plasmid

   #231570

   Purpose

   Expresses the single component, light-off regulated pcVP16-1 transcriptional regulator

   Depositor

   Dominik Niopek

   Article

   Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

   Insert

   Gal4-pcVP16-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMVRha

   Plasmid

   #229553

   Purpose

   Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.

   Depositor

   Sandy Schmidt

   Article

   Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3

   Plasmid

   #231568

   Purpose

   Expresses components of the LOOMINA optogenetic transcriptional control system.

   Depositor

   Dominik Niopek

   Article

   Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

   Insert

   All-in-one LOOMINA construct
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CMV_dCas9-AsLOV2x3-NLSx3

   Plasmid

   #231567

   Purpose

   Expresses components of the LOOMINA optogenetic transcriptional control system.

   Depositor

   Dominik Niopek

   Article

   Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

   Insert

   dCas9-AsLOV2x3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PAC_E83_LOV_eGFP

   Plasmid

   #231574

   Purpose

   This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JD73

    Plasmid

    #229482

    Purpose

    Adenoviral VA RNA I & II genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    VA RNA I & II
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    wt promoter

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. JD81

    Plasmid

    #229481

    Purpose

    Adenoviral VA RNA II gene

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    VA RNA II
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    wt promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. JD92

    Plasmid

    #229478

    Purpose

    CMV promoter driving expression of adenoviral E4orf6 gene

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E4orf6
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGGC018

    Plasmid

    #228232

    Purpose

    Level 0 for LhG4 (GreenGate C module)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    GR-LhG4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    -

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FUS-miRFP670-Coilin

    Plasmid

    #227386

    Purpose

    Adhesion module, creates 'seed' at Cajal body

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    Cajal body FUS IDR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sgTelo

    Plasmid

    #227392

    Purpose

    Adhesion module, guide RNA recognizes telomeres

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    guide RNA targeting telomeres
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. sgPPP1R2

    Plasmid

    #227391

    Purpose

    Adhesion module, guide RNA recognizes PPP1R2

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    guide RNA targeting PPP1R2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJB68

    Plasmid

    #226315

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with Y to A CsoS2 MR1-7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y300A, Y359A, Y420A, Y475A, Y534A, Y585A, Y644A

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJB51

    Plasmid

    #226308

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with V(T/S)G to AAA CsoS2 MR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    VTG273-275AAA, VTG284-286AAA, VTG295-297AAA

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJB48

    Plasmid

    #226307

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with C to S CsoS2 MR1-6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJB47

    Plasmid

    #226306

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with C to S CsoS2 MR1-5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge