We narrowed to 4,679 results for: cre encoding plasmid

1. pFM041

   Plasmid

   #40144

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (S241A) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   S241A; base pairs 6-523 of par-2 were recoded to…

   Promoter

   pie-1

   Available since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pFM039

   Plasmid

   #40142

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   R183-5A + S241A; base pairs 6-523 of par-2 were …

   Promoter

   pie-1

   Available since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pFM036

   Plasmid

   #40139

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (K162A) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   K162A; base pairs 6-523 of par-2 were recoded to…

   Promoter

   pie-1

   Available since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pFM052

   Plasmid

   #40146

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (C56S) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   C56S; base pairs 6-523 of par-2 were recoded to …

   Promoter

   pie-1

   Available since

   Sept. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pFM037

   Plasmid

   #40140

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (R163A) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   R163A; base pairs 6-523 of par-2 were recoded to…

   Promoter

   pie-1

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

   Plasmid

   #203911

   Purpose

   Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   ZF9LOV[C2]-VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

   Plasmid

   #203910

   Purpose

   Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   ZF9LOV[C1]-VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc

   Plasmid

   #138368

   Purpose

   Lentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Proinsulin-NanoLuc in pLX304

   Plasmid

   #62057

   Purpose

   Luminescent reporter of insulin secretion

   Depositor

   David Altshuler

   Article

   Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

   Insert

   Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Inserted NanoLuc into C-peptide of mouse Ins2 cod…

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

    Plasmid

    #115518

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    eGFP
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid

    #112668

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZ:F3-P CAGGS tdTPH-F

    Plasmid

    #112667

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZac2.1-GfaABC1D -IgK- TurboID- NCAN ELS-HA

    Plasmid

    #219809

    Purpose

    Secreted HA-tagged TurboID-NCAN-ELS domain for extracellular proximity labeling

    Depositor

    Cagla Eroglu

    Article

    Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

    Insert

    GfaABC1D
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZac2.1-GfaABC1D -IgK-NCAN ELS-HA

    Plasmid

    #219811

    Purpose

    Secreted HA-tagged NCAN-ELS domain

    Depositor

    Cagla Eroglu

    Article

    Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

    Insert

    IgK (Igk Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBR322-MCK1.35lux

    Plasmid

    #53368

    Purpose

    expresses luciferase under 1.35 kb muscle creatine kinase promoter/enhancer

    Depositor

    Josephine Nalbantoglu

    Article

    Larochelle et al Gene Ther. 1997 May;4(5):465-72.

    Insert

    muscle creatine kinase promoter/enhancer 1.35kb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pREMI-ZA

    Plasmid

    #183736

    Purpose

    Plasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffii

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    Sh ble
    

    Use

    Random insertional gene deletion in komagataella …

    Tags

    Expression

    Mutation

    Promoter

    TEF1 promoter

    Available since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZac2.1-GfaABC1D -IgK- TurboID- NCAN IL-HA

    Plasmid

    #219808

    Purpose

    Secreted HA-tagged TurboID-NCAN-IL domain for extracellular proximity labeling

    Depositor

    Cagla Eroglu

    Article

    Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

    Insert

    IgK (Igk Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)

    Plasmid

    #192190

    Purpose

    HER2 CAR AAV vector PRODH2 KI (pLY080b)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    HER2 CAR AAV vector PRODH2 KI (pLY080b) (ERBB2 Synthetic)
    

    Use

    AAV; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

    Plasmid

    #169191

    Purpose

    To express nsp10-nsp16 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

    Insert

    14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid

    #169177

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells

    Depositor

    John Diffley

    Article

    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515

    Insert

    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag-His6 (nsp5-cleavable)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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