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pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p)

Plasmid

#70197

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Use

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-14

Plasmid

#22763

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 14 (mir-14 primary transcript, Fly)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-9b

Plasmid

#22710

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro RNA 9b (mir-9b primary transcript, Fly)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-315

Plasmid

#22765

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro-RNA 315 (mir-315 primary transcript, Fly)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-92b

Plasmid

#22709

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro-RNA-92b (mir-92b primary transcript, Fly)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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PTet-F30-Pepper aptamer-UTR1-mTurq2

Plasmid

#227645

Purpose

pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

Depositor

Richard Murray

Article

Kapasiawala et al bioRxiv 2023.03.22.533877

Insert

F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL452

Plasmid

#219502

Purpose

Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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Vd-ARATR-GFP

Plasmid

#208903

Purpose

encodes the arachnotocin receptor (oxytocin/vasopressin-like) from the mite Varroa destructor, tagged with GFP

Depositor

Christian Gruber

Article

Duerrauer et al Sci Rep. 2019 Dec 17;9(1):19295. doi: 10

Insert

ARATR

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvH-CtPAN3_448-555_Y

Plasmid

#148093

Purpose

Bacterial Expression of CtPAN3_448-555

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

CtPAN3_448-555

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTet-Bxb1-Xis-mScarlet

Plasmid

#195570

Purpose

Expresses Bxb1 excisionase fused with mScarlet for fluorescence

Depositor

Richard Murray

Article

Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

Insert

Bxb1 Excisionase Fused With mScarlet

Use

Synthetic Biology

Tags

mScarlet

Expression

Mutation

Promoter

P7

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pTet-Bxb1-CFP

Plasmid

#195374

Purpose

Expresses Bxb1 integrase fused with CFP for fluorescence

Depositor

Richard Murray

Article

Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

Insert

Bxb1 Integrase Fused With deCFP

Use

Synthetic Biology

Tags

deCFP

Expression

Mutation

Promoter

pTet

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF_331-374-GST-V5His6_AH

Plasmid

#148934

Purpose

Insect Expression of DmGIGYF_331-374

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_331-374 (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4EHP_AH

Plasmid

#148914

Purpose

Insect Expression of Dm4EHP

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

Dm4EHP (4EHP Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmGIGYF-dsRNAres_AH

Plasmid

#148928

Purpose

Insect Expression of DmGIGYF1-dsRNAres

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF1-dsRNAres (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4EHP_AH

Plasmid

#148930

Purpose

Insect Expression of Dm4EHP

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

Dm4EHP (4EHP Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF-Y58AY60AM65A_AH

Plasmid

#148907

Purpose

Insect Expression of DmGIGYF

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-ScEap1p_101-122-GB1_AG

Plasmid

#148811

Purpose

Bacterial Expression of ScEap1p_101-122

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_101-122 (EAP1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-ScEap1p_107-122-Y107IA108KS110T-GB1_AG

Plasmid

#148813

Purpose

Bacterial Expression of ScEap1p_107-122-Y107IA108KS110T

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_107-122-Y107IA108KS110T (EAP1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-ScEap1p_123-150-GB1_AG

Plasmid

#148814

Purpose

Bacterial Expression of ScEap1p_123-150

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_123-150 (EAP1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-SceIF4G_448-500-GB1_AF

Plasmid

#148716

Purpose

Bacterial Expression of SceIF4G_448-500

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

SceIF4G_448-500 (TIF4631 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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