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pT7-TaGu-ENT-3xF-ORF

Plasmid

#238488

Purpose

For production of C-term flag-tagged TaGu EN-tuned ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Publication

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Mutation

R1109->A

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-TaGu-WT-3xF-ORF

Plasmid

#238487

Purpose

For production of C-term flag-tagged TaGu R2 ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Publication

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-mCherry-RAB7

Plasmid

#221555

Purpose

Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Publication

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEK39

Plasmid

#210599

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Publication

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter

Tags

mRuby3

Expression

Bacterial and Yeast

Promoter

SpH2Bpro

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTU1-A-SP44-mVenus-I

Plasmid

#163763

Purpose

Expresses Streptomyces codon-optimised mVenus-I

Depositor

Paul Freemont, Simon Moore

Publication

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

mVenus-I

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

SP44

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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sgRNA AAVS1

Plasmid

#128119

Purpose

Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Publication

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA AAVS1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTW195b63

Plasmid

#136931

Purpose

Directed evolution of SpCas11

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII(N)-npuN

Use

CRISPR

Expression

Bacterial

Mutation

none

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-RPL23a-C1

Plasmid

#134535

Purpose

Expresses YFP-RPL23a fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RPL23a (RPL23A Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YFP-Rpp21-C1

Plasmid

#134542

Purpose

Expresses YFP-Rpp21 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rpp21 (RPP21 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YFP-Rpp29-C1

Plasmid

#134545

Purpose

Expresses YFP-Rpp29 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rpp29 (POP4 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp38-C1

Plasmid

#134548

Purpose

Expresses YFP-Rpp38 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rpp38 (RPP38 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-FullLength-TET1-LLAA(20)

Plasmid

#124397

Purpose

Full-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cells

Depositor

Shaun Cowley

Publication

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

FLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L897A/L900A mutations (full length protein is 213…

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP Δgag/pol

Plasmid

#101343

Purpose

Deletion from the start of gag until 229 bases before the cPPT

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta GagPol

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSMF2

Plasmid

#112819

Purpose

Expresses wild-type Rpb1 in yeast cells

Depositor

Stephen Fuchs

Publication

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MBD3L2

Plasmid

#106332

Purpose

All-in-one lentivector with tet-inducible MBD3L2

Depositor

Stephen Tapscott

Publication

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

methyl-CpG binding domain protein 3 like 2 (MBD3L2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

tight TRE promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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ABCA1 promoter luciferase reporter (P2 fragment)

Plasmid

#86444

Purpose

Human ABCA1 promoter luciferase reporter (P2 fragment)

Depositor

David Mu

Publication

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Human ABCA1 (ABCA1 Human)

Use

Luciferase

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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ABCA1 promoter luciferase reporter (P3 fragment)

Plasmid

#86445

Purpose

Human ABCA1 promoter luciferase reporter (P3 fragment)

Depositor

David Mu

Publication

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Human ABCA1 (ABCA1 Human)

Use

Luciferase

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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MLF2-GFP-3xFLAG

Plasmid

#230986

Purpose

Expresses GFP tagged MLF2 in mammalian cells

Depositor

Christian Schlieker

Publication

Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MLF2 (MLF2 Human)

Tags

3xFLAG and GFP

Expression

Mammalian

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD55-T2A-GFP

Plasmid

#205450

Purpose

lentiviral plasmid for expression of mouse CD55

Depositor

Deepta Bhattacharya

Publication

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCD55 (Cd55 Mouse)

Expression

Mammalian

Promoter

UBC

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRepair-SGFP2-CTNNB1

Plasmid

#153430

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2

Depositor

Renee van Amerongen

Publication

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SGFP2

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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