We narrowed to 15,871 results for: nans

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Anti-Pan-Ankyrin [N388A/60R]

Plasmid

#128640

Purpose

Mammalian Expression Plasmid of anti-Pan-Ankyrin. Derived from hybridoma N388A/60.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Ankyrin Pan recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pN1 VAMP2 R125TAG

Plasmid

#69877

Purpose

express in mammalian cells for unnatural amino acid incorporation into VAMP2

Depositor

Silvio Rizzoli

Article

Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

Insert

VAMP2 (Vamp2 Rat)

Use

Synthetic Biology

Expression

Mammalian

Mutation

Amber and Ochre stop codons in the former linker …

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
Anti-SVOP [N356/23R]

Plasmid

#114499

Purpose

Mammalian Expression Plasmid of anti-SVOP (Rat). Derived from hybridoma N356/23.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SVOP (Rattus norvegicus) recombinant mouse monoclonal antibody (Svop Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-SCG10/Stathmin-2 [L5/1R]

Plasmid

#140065

Purpose

Mammalian Expression Plasmid of anti-SCG10/Stathmin-2 (Rat). Derived from hybridoma L5/1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SCG10/Stathmin-2 (Rat) recombinant mouse monoclonal antibody (Stmn2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-nAChr-beta3

Plasmid

#48811

Purpose

This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

Depositor

Henry Lester, Jerry Stitzel

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)

Expression

Mammalian

Mutation

*

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-FHF-A [N235/22R]

Plasmid

#114509

Purpose

Mammalian Expression Plasmid of anti-Pan-FHF-A (Human). Derived from hybridoma N235/22.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-FHF-A (Homo sapiens) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

dual CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4 Cluster Promoter Delta 1.2

Plasmid

#100130

Purpose

It contains 0.5 Kb of the miR-183-96-182 cluster promoter. This fragment partially loses promoter activity in melanoma cells.

Depositor

Eva Hernando

Article

Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

Insert

miR-183-96-82 Cluster promoter 0.5 Kb Fragment (MIR182 Human)

Use

Luciferase

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4-Cluster Promoter Delta 1.1

Plasmid

#100129

Purpose

It contains ~0.8 Kb of the miR-183-96-182 cluster promoter. Is the minimal region that contains the maximal promoter activity

Depositor

Eva Hernando

Article

Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

Insert

miR-183-96-82 Cluster promoter 0.8 Kb Fragment (MIR182 Human)

Use

Luciferase

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Pannexin-2 [N121A/31R]

Plasmid

#140073

Purpose

Mammalian Expression Plasmid of anti-Pannexin-2 (Rat). Derived from hybridoma N121A/31.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pannexin-2 (Rat) recombinant mouse monoclonal antibody (Panx2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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C-His-GvpC-Calpain

Plasmid

#153295

Purpose

Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Calpain-sensitive Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

1) Contains an extra glycine after the methionine…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna3-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128338

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna3 (Chrna3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128337

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna2 (Chrna2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna4-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128339

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna4 (Chrna4 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrnb3-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128344

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrnb3 (Chrnb3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-10 [N269/73R]

Plasmid

#114558

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-10 (Mouse). Derived from hybridoma N269/73.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-10 (Mus musculus) recombinant mouse monoclonal antibody (Syt10 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCIN4-EZOD

Plasmid

#44977

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2 DBD (Ets2 Mouse)

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

Ets2 DNA binding domain (AA 332-468) only = domin…

Promoter

CMV

Available Since

May 28, 2013

Availability

Academic Institutions and Nonprofits only
L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

Plasmid

#222876

Purpose

Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

FRB chain: V84R in GPA

Promoter

MSCV LTR

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_H6_His

Plasmid

#201719

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH_H6 (S )

Tags

Hexahistidine

Expression

Bacterial

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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Kv2.1 K+ channel scFv [D4/154]

Plasmid

#206787

Purpose

Mammalian Expression of Kv2.1 K+ channel scFV. Derived from hybridoma D4/154 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv2.1 K+ channel recombinant scFV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pVITRO1-HAPPID1

Plasmid

#204626

Purpose

Expresses the human anti-Phl p 7 IgD/λ antibody 102.1F10 (HAPPID1) in mammalian cells. HAPPID1 binds to the grass pollen allergen Phl p 7 with subnanomolar affinity.

Depositor

James McDonnell

Article

Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75

Inserts

HAPPID1 heavy chain
HAPPID1 light chain

Expression

Mammalian

Promoter

mouse elongation factor 1 alpha and rat elongatio…

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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