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pRAR120

Plasmid

#185041

Purpose

J23108 promoter, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Only first 90 nt of rpmE coding present

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG/HA-DCP1

Plasmid

#215843

Purpose

Expression of FLAG/HA-DCP1

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human DCP1 (DCP1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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LLP782_Traptavidin-KRAB-CO

Plasmid

#211775

Purpose

AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-DNMT3A

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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LLP785_Traptavidin-DNMT3A-CO

Plasmid

#211778

Purpose

AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-EZH2

Use

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP781_SnoopCatcher-KRAB-CO

Plasmid

#211774

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-KRAB

Use

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC7

Plasmid

#205060

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Use

Tags

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 7 aa

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC13

Plasmid

#205063

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Use

Tags

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 13 aa

Promoter

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-mSox2C17

Plasmid

#206369

Purpose

Expresses mouse SOX2-C17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox2-C17 chimera (Sox2, Sox17 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC17

Plasmid

#205061

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Use

Tags

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 17 aa

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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HA-Halo-H2DBD

Plasmid

#200206

Purpose

Expression of truncated HSF2 (DBD)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HA-Halo-H2DBD (Hsf2 Mouse)

Use

Tags

Halo; HA

Expression

Mammalian

Mutation

HSF2 (1-366; 15 bp linker; 972-1017)

Promoter

EF_1alpha

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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H1DBD-Halo-HA

Plasmid

#200203

Purpose

Expression of truncated HSF1 (DBD and HR)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

H1DBD-Halo-HA (Hsf1 Mouse)

Use

Tags

Halo; HA

Expression

Mammalian

Mutation

HSF1 (1-399)

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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H1DBD-HR-Halo

Plasmid

#200204

Purpose

Expression of truncated HSF1 (DBD and HR)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

H1DBD-HR-Halo (Hsf1 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

HSF1 (1-672)

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

Plasmid

#199254

Purpose

Expression construct encoding the high specificity SpCas9-KARA-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity S. pyogenes Cas9-D10A-KARA nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A R1060A

Promoter

CAG promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

Plasmid

#199253

Purpose

Expression construct encoding the high specificity SpCas9-KA-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity S. pyogenes Cas9-D10A-KA nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A

Promoter

CAG promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSUPERpuro-BglII-HsEDC4-t1_V

Plasmid

#147796

Purpose

Mammalian Expression of HsEDC4-t1-shRNA

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsEDC4-t1-shRNA (EDC4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP2_1-395_V

Plasmid

#147791

Purpose

Mammalian Expression of HsDCP2_1-395

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_1-395 (DCP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A_T

Plasmid

#147636

Purpose

Insect Expression of HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A (TNRC6C Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6A_1209-1709_S

Plasmid

#147520

Purpose

Bacterial Expression of HsTNRC6A_1209-1709

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709 (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6A_1209-1709-CIM1mut_S

Plasmid

#147523

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-CIM1mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-CIM1mut (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6A_1209-1709-CIM2mut_S

Plasmid

#147524

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-CIM2mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-CIM2mut (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge